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Öğe 4-(3,4-dihydroxybenzoyloxymethyl)phenyl-O-beta-D-glucopyranoside effect in liver regeneration(F Hernandez, 2019) Fırat, Tülin; Söyler, Gizem; Töre, Fatma; Şit, Mustafa; Kıyan, Aysu; Özgen, Ufuk; Kükner, Aysel; Şit, MustafaFollowing an injury or resection, the mammalian liver has the capacity to regain its former volume and functioning by restoring itself. Studies have demonstrated that antioxidants play a role in hepatic regeneration. This study investigated the effect of 4-(3,4-dihydroxybenzoyloxymethyl)phenyl-O-beta-D-glucopyranoside (PG) obtained from Origanum micranthum on liver regeneration. Sixty Wistar Albino rats were used. In the sham-operated group, a midline abdominal laparotomy was performed without hepatectomy. In the partial hepatectomy (PHx) group, the median and left lateral lobes were removed. Rats in the PHx group received 20 mg/kg/day PG intraperitoneally before being sacrificed at 24, 48, and 72 hrs, and 7 days later. Liver tissues were collected for immunohistochemical analysis and electron microscopic evaluation. We found an increase in mitotic index, and the numbers of Ki-67 stained hepatocytes in all PHx early stage groups (24 hr, 48hr, 72 hr), but not in 7-day groups. The regeneration mediators eNOS, iNOS, TNF-alpha and NF-kappa B were shown to increase in PHx groups. This increase was more prominent dependening on time. In the PHx treatment (PHx+PG) groups, while eNOS was still high, iNOS, TNF-alpha and NF-kappa B had decreased. The apoptotic index was markedly high in the PHx groups; this was prevented by PG treatment. These findings were supported by the ultrastructural results. Our findings indicate that PG supports liver regeneration, hepatocyte proliferation, reduced liver damage, and inflammatory mediators following PHx.Öğe 7-Ethoxyresorufin O-deethylase and glutathione S-transferase activities of rats treated with morin, 7,12-dimethylbenz[a] anthracene and endosulfan(Wiley-Blackwell, 2016) Sapmaz, Canan; Fırat, Tülin; Kükner, Aysel; Bozcaarmutlu, Azra[No Abstract Available]Öğe Alkoxyresorufin O-dealkylase activities in rats treated with 7,12-dimethylbenz[a]anthracene and endosulfan(Wiley-Blackwell, 2015) Sapmaz, Canan; Fırat, Tülin; Kükner, Aysel; Bozcaarmutlu, AzraÖğe Alteration in xenobiotic metabolizing enzyme activities with morin and 7,12-dimethylbenz(a) anthracene in diabetic male rats(Wiley-Blackwell, 2015) Sapmaz, Canan; Fırat, Tülin; Kükner, Aysel; Bozcaarmutlu, AzraÖğe Carvacrol treatment opens Kir6.2 ATP-dependent potassium channels and prevents apoptosis on rat testis following ischemia-reperfusion injury model(EDITURA ACAD ROMANE, 2021) Balcı, Cemre Nur; Fırat, Tülin; Acar, Nuray; Kükner, AyselTesticular torsion is a urological problem that causes subfertility and testicular damage in males. Testis torsion and detorsion lead to ischemia– reperfusion (IR) injury in the testis. Testicular IR injury causes the increase of reactive oxygen species (ROS), oxidative stress (OS) and germ cell-specific apoptosis. In this study, we aimed to investigate whether Carvacrol has a protective effect on testicular IR injury and its effects on Kir6.2 channels, which is a member of adenosine triphosphate (ATP)-dependent potassium channels. In the study, 2–4 months old 36 albino Wistar rats were used. For experimental testicular IR model, the left testis was rotated counterclockwise at 720º for two hours, and after two hours following torsion, detorsion was performed. Carvacrol was dissolved in 5% Dimethyl Sulfoxide (DMSO) at a dose of 73 mg/kg and half an hour before detorsion, 0.2 mL was administered intraperitoneally. In testicular tissues, caspase 3 and Kir6.2 immunoexpressions were examined. Serum malondialdehyde (MDA) and testosterone levels were measured. Apoptotic cells and serum MDA levels were significantly decreased and Kir6.2 activation was significantly increased in Carvacrol-administrated IR group. As a result of our study, Carvacrol may activates Kir6.2 channels and inhibits apoptosis and may have a protective effect on testicular IR injury.Öğe Çinkodan zengin diyetin, sigara dumanı maruziyetindeki sıçan mesane dokusu sitokin profiline etkisi(2015) Hakan, Elçin Terzi; Kükner, Aysel; Aydın, Hatip; Özyalvaçlı, Mehmet Emin; Fırat, Tülin; Üyetürk, UğurAmaç: Sigara içimi akciğer, larinks, özefagus, mide, mes ane ve böbrek kanserleri ile kronik hastalıkların sebebidir. Sitokinler, inflamasyonun başlatılması ve sürdürülmesinde önemlidirler. Sigara, çinko eksikliği sebebidir. Çinko, antioksidan olup serbest radikallerin zararlarından hücreleri korur. Çalışmamızda sigara dumanına maruz bırakılarak çinkodan zengin diyet ile beslenen sıçanların mesane dokusundaki TLR 2, TLR 4, Nükleer faktör ka ppa B, İnterl ökin 1? ve İnterlökin 10 ekspresyonuna etkisi incelenmesi amaçlanmıştır. Yöntem : Çalışmada 24 adet erişkin erkek Wistar-Albino sıçanlar kullanıldı. Sıçanlar 4 gruba ayrıldı. Birinci grup normal, 2. grup ise çinkodan zengin diyetle beslendi. Üçüncü ve 4. gruplar sigara inhalasyonu yapılan gruplar olup sırasıyla normal ve çinkodan zengin diyetle beslenen gruplardı. Den ey sonunda alınan mesane dokuları histol ojik olarak değerlendirildi. Kalan doku örnekleri ise Real time polymerase chain reaction yöntemi ile sitokin ekspresyonları gösterilerek değerlendirildi. Bulgular: Çalışmadaki hiçbir grup mesane epitelinde değ işikli k izlenmemiştir. Sigara verilen grupta sadece düz kas yapılarında tabakalar arasında ödem ve bazı alanlarda yoğun inflamatuar hücreler ile bu alanlarda hemoraji ve konjesyona rastlanılmıştır. Çinkodan zengin diyetle besl enen denekler de ise inflamatuar hüc relere, konjesyona ve ödeme daha az rastlandı. Tüm sıçan gruplarının mesane dokularında sitokin ekspresyonları ikili olarak karşılaştı rmalarda sigaraya maruz kalmış sıçanlarda çinko almayan ve çinko alan sıçanlar için NF -?B2 geni için anlamlı farklılık bul undu. Sonuç : Sonuç olarak sigara maruz kalan sıçanlarda çinko kullanımının anlamı bir şekilde NF -?B2 ekpresyonunu artışına neden olması, çinkonun sigaraya bağlı gelişen mesane kanserlerinde kanserli hücre apopitozunu arttırarak tedaviye katkı yapabileceğin i düşündürmektedir.Öğe Comparison of age-related anti müllerian hormone, inhibin-b levels and follicle reserve in rat ovarium(Universidad de la Frontera, 2021) Söyler, Gizem; Bugdayci, Güler; Kükner, AyselAnti-Müllerian hormone (AMH) and Inhibin B (INHB) in the glycoprotein structure are members of the transforming growth factor ? family and expressed by granulosa cells from puberty. AMH is a factor that increases the life span of small developing follicles. For this reason, it is widely used to determine the ovarian reserve and age. Inhibin-B secreted from granulosa cells plays a role in regulation of the Follicle Stimulating Factor (FSH) and determination of the follicle diameter. There are few studies on the effect of these two age-related hormones on ovarian histology in rats. In this study, AMH and INHB expression in ovarian tissues of female rats of different age groups, their relationship with ovarian structure and folliculogenesis were examined histologically and biochemically. Wistar Albino rats were used in the study and a total of 3 groups were formed. The ovaries of rats in the pre-oestrous period were collected, and follicle count was performed on tissue sections in batches. Expression of AMH in the follicles was identified immunohistochemically. In serum, AMH and INHB levels were assessed by ELISA method and their significance was evaluated statistically. Results from light microscopic examination determined that AMH was expressed from the granulosa cells of developing follicles. INHB expression during the prepubertal period and AMH had a protective effect on the ovarian reserve and the number of developing follicles, respectively. © 2021, Universidad de la Frontera. All rights reserved.Öğe Comparison of age-related anti mullerian hormone, ınhibin-b levels and follicle reserve in rat ovarium(SOC CHILENA ANATOMIA, 2021) Söyler, Gizem; Buğdaycı, Güler; Kükner, AyselAnti-Mullerian hormone (AMH) and Inhibin B (INHB) in the glycoprotein structure are members of the transforming growth factor beta family and expressed by granulosa cells from puberty. AMH is a factor that increases the life span of small developing follicles. For this reason, it is widely used to determine the ovarian reserve and age. Inhibin-B secreted from granulosa cells plays a role in regulation of the Follicle Stimulating Factor (FSH) and determination of the follicle diameter. There are few studies on the effect of these two age-related hormones on ovarian histology in rats. In this study, AMH and INHB expression in ovarian tissues of female rats of different age groups, their relationship with ovarian structure and folliculogenesis were examined histologically and biochemically. Wistar Albino rats were used in the study and a total of 3 groups were formed. The ovaries of rats in the pre-oestrous period were collected, and follicle count was performed on tissue sections in batches. Expression of AMH in the follicles was identified immunohistochemically. In serum, AMH and INHB levels were assessed by ELISA method and their significance was evaluated statistically. Results from light microscopic examination determined that AMH was expressed from the granulosa cells of developing follicles. INHB expression during the prepubertal period and AMH had a protective effect on the ovarian reserve and the number of developing follicles, respectively.Öğe Comparison of ocular surface side effects of topical travoprost and bimatoprost(Karger, 2008) Alagöz, Gürsoy; Bayer, Atilla; Boran, Çetin; Serin, Didem; Kükner, Aysel; Elçioğlu, MustafaPurpose: To compare the subjective symptoms, conjunctival hyperemia, tearing response and conjunctival cytological changes secondary to topical administration of bimatoprost and travoprost for 6 months. Methods: Newly diagnosed primary open-angle glaucoma patients were randomly prescribed bimatoprost (35 cases) or travoprost (42 cases). Two patients in each group were excluded because they did not appear at their appointments regularly. Thus, 33 and 40 patients completed the study in the bimatoprost and travoprost groups, respectively. Redness, itching, foreign-body sensation, pain and discomfort were assessed by a questionnaire, and patients were examined for conjunctival hyperemia. Schirmer's I and break-up time tests were performed, and impression cytology of conjunctiva was evaluated. Results: Subjective symptoms were similar in both groups. The only subjective symptom that changed significantly was redness. The change in conjunctival hyperemia along the study period correlated with the patient-reported redness in both groups, being highest on day 30. Schirmer's test I and break-up time did not change with time and were similar in both groups. The impression cytology grade increased with time in both groups with the only significant difference between groups on day 90 (higher in the bimatoprost group). Conclusion: We observed conjunctival hyperemia as the most common side effect of bimatoprost and travoprost. Tear film functions were not affected by these drugs while cytological alterations were. Copyright (C) 2008 S. Karger AG, Basel.Öğe Cytotoxic effects of nasal buserelin on nasal mucosal tissue in rabbits(Springer, 2012) Oğhan, Fatih; Apuhan, Tayfun; Terzi, Hakan; Kükner, Aysel; Çoksuer, Hakan; Yılmaz, FahrettinTo investigate the cytotoxic effects of nasal buserelin on rabbit nasal mucosal tissue, twenty-four female rabbits were studied prospectively. The rabbits were divided into 4 groups including 6 rabbits. The rabbits' left noses were included in the all study groups: 150 mu g/puff/day of buserelin acetate was administered topically twice daily during 21, 42 and 63 days. Saline was administered topically twice daily to the left nasal cavity in the control group. The nasal septal mucosal stripe tissue was carefully removed from underlaying cartilage after sedation. HE staining, Masson's trichrome, toluidine blue and TUNEL staining were used to evaluate mucosal changes. Each preparation was investigated via apoptotic cells, and they were accounted. Kruskal-Wallis test was used to evaluate nonparametric comparison of apoptotic cells. Mononuclear cells have been raised in the sub-epithelial connective tissue, nucleuses of epithelial cells in the apical region were pyknotic, and apoptotic cells were determined on 21-day group. In the 42-day group, nasal epithelial tissue was similar to 21-day group and epithelial cells including pyknotic nucleus were present in this group, too. In the 63-day group, epithelial cells were light colored. Venous sinuses in the sub-epithelial connective tissue were wide but not congested and not raised collagen filaments. In the intra-epithelial tissue, some of cells were TUNEL (+). Apoptotic cells were fewer in the control group according to 21-day group. In 42- and 63-day groups, these cells were fewer than in 21-day group. Numerical difference was present between the groups, but statistical significance was not found between the groups. We concluded that nasal buserelin cytotoxicity was not potent in the nasal cavity in rabbits. We use nasal buserelin in all indications with confidence.Öğe Deneysel karaciğer hasarının akciğer dokusuna etkisi ve mast hücrelerinin rolü(Türkiye Klinikleri Tıp Bilimleri Dergisi, 2013) Fırat, Tülin; Ulaş, Nilüfer; Terzi, E. Hakan; Töre, Fatma; Kükner, AyselAmaç: Deneysel karaciğer hasarına bağlı oluşan akciğer dokusu ve mast hücre sayısındaki değişikliklerin ışık mikroskobik olarak incelenmesi. Gereç ve Yöntemler: Bu çalışmada yetişkin Wistar albino ratlar kullanıldı. Ratlar rastgele, her grupta 6 hayvan olmak üzere 3 gruba ayrıldı. Kontrol grubu, CCL4 grubu ve ligasyon grubu. CCL4 grubunda, CCl4 (1 mL/kg vücut ağırlığı, intraperitoneal), haftada 2 kez toplam 8 hafta uygulandı. Ligasyon grubu, ortak safra kanalı bağlanarak 3 hafta bekletildi. Karaciğer ve akciğer dokularından alınan örnekler hematoksilen-eozin ile boyandı. Akciğer kesitleri toluidin mavisi ile boyanarak parankim ve plevral mast hücreleri sayıldı. Bulgular: Kanal ligasyonu ve CCl4 uygulanan grupların akciğer dokularında interalveolar septum kalınlaşması, kapiller konjesyon, ödem, artmış inflamatuar hücreler ve degranüle mast hücresi tespit edildi. Ligasyon grubunda ise akciğer dokularında bu değişiklikler daha belirgindi. Parankimal ve plevral mast hücre sayısı ligasyon ve CCl4 grubunda kontrole göre anlamlı olarak artmıştı. Sonuç: Hepatopulmoner sendrom olarak tanımlanan karaciğer hasarının neden olduğu akciğer hasarına, artmış mast hücrelerinin aracılık edebileceği tespit edilmiştir.Öğe Dietary vitamin C and E modulates oxidative stress induced-kidney and lens injury in diabetic aged male rats through modulating glucose homeostasis and antioxidant systems(Wiley, 2011) Özkaya, Dilek; Nazıroğlu, Mustafa; Armağan, Abdullah; Demirel, Alpay; Köroğlu, Banu Kale; Kükner, AyselDiabetes induces oxidative stress in aged human and rat, although daily supplementation of vitamins C and E (VCE) can be beneficial to aged diabetic rats by reducing free radical production. The aim of the present study was to evaluate whether dietary VCE supplementation relieves oxidative stress in streptozotocin (STZ)-induced diabetic in aged rats. Thirty aged rats were randomly divided into three groups. The first group was used as a control. The second group was made diabetic using a single dose of intraperitoneal STZ. VCE-supplemented feed was given to aged diabetic rats constituting the third group. On the 21st day of the experiment, blood, lens and kidney samples were taken from all animals. Glutathione peroxidase (GSH-Px) activity in lens and kidney, reduced glutathione (GSH), vitamin E and beta-carotene concentrations in kidney were lower in the diabetic group than in the control whereas plasma glucose, urea and creatinine, and kidney and lens peroxidation (LP) levels were higher in the diabetic group than in the control. However, kidney and lens LP levels, and plasma glucose, urea and creatinine values were decreased by VCE supplementation. Lens and kidney GSH-Px activity, kidney GSH, vitamin E and beta-carotene concentrations and erythrocyte counts were increased by VCE treatment. Kidney weights, vitamin A, haemoglobin, hematocrit, leukocyte and platelets values were not changed by diabetes and/or VCE supplementation. VCE ameliorated also diabetes-induced histopathological changes in kidney. In conclusion, we observed that VCE supplementation is beneficial towards kidney and lens of aged diabetic rats by modulating oxidative and antioxidant systems. Copyright (C) 2011 John Wiley & Sons, Ltd.Öğe Does platelet-rich plasma enhance micro fracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model(Turkish Assoc Orthopaedics Traumatology, 2013) Hapa, Onur; Çakıcı, Hüsamettin; Yüksel, Halil Yalçın; Fırat, Tülin; Kükner, AyselObjective: The purpose of the present study was to compare the effectiveness of platelet-rich plasma (PRP) + microfracture and microfracture treatments in the healing of chronic focal chondral defects. Methods: The study included 57 adult male Sprague-Dawley rats. Forty-two rats were divided into three groups of 14 rats with a chondral defect (control, microfracture only, PRP+microfracture). The remaining 15 rats were used to produce the PRP preparation. The rats were then euthanatized at 3 and 6 weeks after treatment and examined. Histological analysis using the modified Pineda scoring system and immunohistochemical staining for Type 2 collagen were performed. Results: At both time intervals, control group histological scores (Week 3: 8.8+/-1.2, Week 6: 8.5+/-0.7) were higher than microfracture (Week 3: 6.8+/-1.0, Week 6: 7.1+/-0.6) and PRP+microfracture (Week 3: 6.4+/-1.3, Week 6: 5.7+/-1.2) scores (p<0.05). The microfracture group score was higher at Week 6 than the PRP+microfracture group (p<0.05). The degree of Type 2 collagen staining was higher at Week 6 in the PRP+microfracture group and was unique in showing staining at the cell membrane. Conclusion: The addition of PRP application to microfracture treatment appears to enhance cartilage healing in chronic focal chondral defects.Öğe Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model(2013) Hapa, Onur; Çakici, Hüsamettin; Yüksel, Halil Yalçın; Firat, Tülin; Kükner, Aysel; Aygün, HayatiAmaç: Mevcut çalışmanın amacı trombositten zengin plazma (platelet-rich plasma, PRP) + mikrofraktür ile mikrofraktür tedavilerinin kronik fokal kondral defekt iyileşmesine etkisini karşılaştırmaktı. Çalışma planı: Bu çalışmada 57 adet erişkin erkek Sprague-Dawley sıçanı kullanıldı. Kırk iki sıçan kondral defekt oluşturulduktan sonra 14’erlikten 3 gruba (kontrol, sadece mikrofraktür, PRP+mikrofraktür) ayrıldı. Kalan 15 sıçan PRP hazırlanmasında kullanıldı. Tedaviden 3 ve 6 hafta sonra sıçanlara ötenazi uygulandı ve incelemeleri yapıldı. Uyarlanmış Pineda skorlama sistemi ile histolojik analiz ve Tip 2 kollajen için immünohistokimyasal boyama yapıldı. Bulgular: Her iki zaman aralığında da, kontrol grubu histolojik skorları (3. hafta: 8.8±1.2, 6. hafta: 8.5±0.7) mikrofraktür (3. hafta: 6.8±1.0, 6. hafta: 7.1±0.6) ve PRP+mikrofraktür (3. hafta: 6.4±1.3, 6. hafta: 5.7±1.2) gruplarından daha yüksekti (p<0.05). Altıncı hafta mikrofraktür grup skoru, 6. hafta PRP+mikrofraktür grubundan daha yüksekti (p<0.05). Tip 2 kollajen boyanma derecesi 6. haftada PRP+mikrofraktür grubunda daha yüksekti ve hücre membranında membranöz boyama gözlendiğinden benzersizdi. Çıkarımlar: Kronik fokal kondral defekt tedavisi için mikrofraktürlerle birlikte PRP uygulamasının kıkırdak iyileşmesini daha etkin kıldığı gözükmektedir.Öğe Effect of beta-glucan in lung damage secondary to experimental obstructive jaundice(Aves, 2012) Erkol, Mehmet Hayri; Kahramansoy, Nurettin; Kordon, Özgür; Büyükaşık, Oktay; Serin, Erdinç; Kükner, AyselBackground/aims: This study aimed at investigating the protective effects of beta-glucans on the lungs in obstructive jaundice. Methods: In total, five groups -Sham (1), control (2) and treatment groups (3,4,5)- were established; each comprising randomly selected seven Wistar Albino rats. Beta-glucan was given after choleduct ligation in Group 3 while it was given before and after the choleduct ligation in Group 4. As pre-treatment beta-glucan was given before ligation in Group 5. Beta-glucan was administered in a single dose of 50 mg / kg / day by gavage for a ten-day period. Superoxide dismutase, and myeloperoxidase levels in serum; malotzdialdehyde, lipid hydroxyperoxidase and glutathione levels in lung tissue; lactate dehydrogenase levels in bronchoalveolar lavage fluid were measured. Results: The blood polymorphonuclear leukocytes level was highest in the control group and lower in the sham and treatment groups. Serum superoxide dismutase and tissue glutathione values were significantly higher in Groups 3 and 4 (p <= 0.04) whilst Groups 3 and 4 did not differ from each other. In Groups 3 and 4 malondialdehyde, lipid hydroxyperoxidase, and myeloperoxidase values were significantly lower. However, Groups 3 and 4 did not differ for malondialdehyde or lipid hydroxyperoxidase values. Lactate dehydrogenase level in the bronchoalveolar lavage fluid was significantly lower in all of the treatment groups (Groups 3,4,5) (p <= 0.008). When compared to the control group, it was observed that lung damage was much more limited in the treatment groups (p<0.001). Conclusion: This study suggests that beta-glucan exhibits protective effect in pulmonary tissue against oxidative damage in obstructive jaundice.Öğe The effect of co-administration of berberine, resveratrol, and glibenclamide on xenobiotic metabolizing enzyme activities in diabetic rat liver(Taylor & Francis Ltd, 2020) Bozcaarmutlu, Azra; Sapmaz, Canan; Bozdoğan, Ömer; Kükner, Aysel; Kılınç, Leyla; Kaya, Salih Tunç; Özarslan, Oğulcan Talat; Ekşioğlu, DidemIt is possible to use plant-derived antioxidant molecules in the form of dietary supplements. However, dietary supplement-drug interaction pattern has not been well defined for most of these products. The aim of this study was to determine the effects of berberine, resveratrol, and glibenclamide on xenobiotic metabolizing enzyme activities in diabetic rats. Streptozotocin was administered to create experimental diabetes. Resveratrol (5 mg/kg) (R), glibenclamide (5 mg/kg) (G), and berberine (10 mg/kg) (B) were administered individually or in combinations in DMSO by intraperitoneal administration route to the diabetic rats. DMSO was also given to non-diabetic control (C) and diabetic control (D) groups. Livers of rats were taken under anesthesia at the end of the treatment period (12 days). Ethoxyresorufin O-deethylase (EROD), pentoxyresorufin O-depentylase (PROD), aniline 4-hydroxylase (A4H), erythromycin N-demethylase (ERND), glutathione S-transferase (GST), catalase (CAT), and glutathione reductase (GR) activities were measured in microsomes and cytosols. In addition, histomorphological studies were also performed in the liver tissues. EROD activity of D+R was significantly higher than C and D+R+B. PROD activity of D+R was significantly higher than C, D, D+R+G, D+R+B, and D+R+B+ G. PROD activity of D+B was significantly higher than C and D+R+B. ERND activity of D+R was significantly higher than D+R+G and D+R+B. GST activity of D+R was significantly higher than D+R+G. CAT activity of D+B was significantly lower than C. It is clear that co-administration of resveratrol, berberine, and glibenclamide modifies some of the important xenobiotic metabolizing enzyme activities. Resveratrol and berberine have the potential to cause dietary supplement-drug interaction.Öğe Effect of co-administration of endosulfan and morin on biomarker enzyme activies in rat liver(Wiley-Blackwell, 2014) Sapmaz, Canan; Fırat, Tülin; Kükner, Aysel; Bozcaarmutlu, AzraÖğe The effect of ethanol intake on tendon healing: a histological and biomechanical study in a rat model(Springer, 2009) Hapa, Onur; Çakıcı, Hüsamettin; Gideroğlu, Kaan; Özturan, Kutay; Kükner, Aysel; Buğdaycı, GülerEthanol has a suppressive effect on inflammation and the immune system, but the effect of ethanol on tendon healing in vivo has not been studied. The purpose of this study was to investigate the histological and biomechanical effects of ethanol intake on tendon healing in a rat tendon injury model. Forty-seven rats were randomly assigned to either ethanol or control groups. Progressively increasing concentrations of ethanol combined with glucose were administered to these rats in their drinking water. After 1 week, the Achilles tendon of each rat was injured proximal to its insertion on the calcaneus. All rats were euthanized at 4 weeks. The tendons were evaluated both histologically and biomechanically. The histologic examination of these tendons was done using a semi-quantitative 4-point scale to rate cell morphology, the degree of ground substance staining, collagen organization, and vascular changes. Load to failure (N) strength was obtained with biomechanical testing. Tendon failure loads were lower in the ethanol group (31.6 +/- A 8.8 N) than in the control group (39.7 +/- A 8.2 N) (P = 0.04). Histologic tenocyte scores were higher in the ethanol group (1.90 +/- A 0.73) than the control group (0.9 +/- A 0.73) (P = 0.01). Ethanol ingestion resulted in abnormal tenocyte morphology, disorganized collagen bundles with a tendency toward increased tenocyte number, and neovascularization 3 weeks after the tendon injury indicating delayed and abnormal healing. The healing tendons in the alcohol treated group failed at statistically lower loads than the control group.Öğe Effect of heparin on inflammation : an animal model of tracheal stents(Wiley-Blackwell, 2014) Biçer, Yusuf Özgür; Köybaşı, Serap; Süslü, Ahmet E.; Kükner, Aysel; Tezcan, ErkanObjectives/Hypothesis: The objective of this study was to investigate the effect of systemic heparin on tracheal and wound healing. Study Design: An animal experiment using a tracheal stent model. Methods: Twenty Wistar albino rats were divided into two groups; a heparin group (n=10) that received 210 U/kg/d heparin sodium, and a saline group (n=10) that received 0.1 mL of 9% NaCl. Stents of 1 cm in length made of 8F feeding tube were placed into the tracheas of rats in both groups and stayed in place for 10 days. Ten days after removal of the stents, the rats were sacrificed and the tracheas were harvested. Histological evaluations of the tracheas were performed with respect to inflammatory parameters. Results: We observed significantly milder inflammation in the heparin group compared to the saline group in terms of inflammatory cell count, fibroblastic proliferation, edema, and vascularity at the site where the tracheal incision was made (P<.05). Inflammation tended to be of a lesser extent in the stent site in the heparin group (P>.05). Conclusions: We could demonstrate that heparin does inhibit fibroblast proliferation, inflammatory cell count, edema, and angiogenesis in this animal model. We believe that future studies can elucidate on laryngotracheal wound healing as well as their molecular mechanisms.Öğe The effect of kisspeptin on spermatogenesis and apoptosis in rats(2017) Aytürk, Nilüfer; Fırat, Tülin; Kükner, Aysel; Özoğul, Candan; Töre, FatmaBackground/aim: To study the effect of kisspeptin, a gonadotropin release stimulator, on the testicular tissue of the rat. Materials and methods: Four groups were formed as follows: control, Kiss-10 50 nmol administration for 1 day, Kiss-10 administration for 13 days, and one last group kept for 7 days following Kiss-10 applied for 13 days. Testicular tissues were stained with hematoxylineosin, periodic acid Schiff, Masson trichrome staining, terminal deoxynucleotidyl transferased UTP nick-end labeling, and Ki-67 immune staining. Serum testosterone levels were determined. Results: Serum testosterone level increased following acute application, while it was reduced by chronic treatment. Spermatogenic cells as stained by Ki-67 and TUNEL increased in the treated groups compared to the controls. Following a 7-day rest after treatment, a decrease in testosterone levels and Ki-67–stained cell numbers and an increase in TUNEL-stained cells were observed. Leydig cells showed increased vacuolization in the Kiss-1 group. Leydig cell vacuolization continued in the Kiss (13) group and was reduced in the Kiss (13 + 7) group. Conclusion: Kiss-10 increased spermatogenic cell proliferation, while testosterone level and proliferation decreased and apoptosis increased during the waiting period.
