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    ANALYSING SUBGINGIVAL PLAQUE WITH REGARD TO H. PYLORI AT CHRONIC AND AGGRESSIVE PERIODONTITIS PATIENTS
    (2018) Ustaoğlu, Gülbahar; Ercan, Esra; Korkmaz, Meltem Zihni; Uzun, Bilge Cansu; Buruk, Celal Kurtuluş
    Amaç:Helicobacter pylori (H. Pylori), bir gram (-), mikroaerofilik bakteri olup, kronik aktif gastrit ve peptik ülserin etyolojik faktörüdür. Bazı çalışmalar, bu bakterinin, oral kavitede bulunduğunda, mide için potansiyel rezervuar olabileceğini göstermiştir. Çeşitli çalışmalar, H. pylori'nin kronik periodontitisli hastaların tükrük ve subgingival plaklarında görülebileceğini periodontitis hastaları ile ilgili herhangi bir veri yoktur. Bu çalışmada, kronik, agresif periodontitis ve gingivitis hastalarının subgingival plak örneklerinde H. pylori prevalansını saptamayı ve hastaların gastrik problemler konusunda bilinçlenmesini arttırmayı amaçladık. Gereç ve Yöntem: Bu çalışma, gastrik hastalık semptomu olmayan ve son 3 ayda antibiyotik kullanmayan 155 hasta (61 adet gingivitis, 60'ı kronik periodontitisli ve 34 agresif periodontitisli) içermekteydi. Subgingival plak örnekleri steril paper point kullanılarak alındı. H. pylori, A. actinomycetemcomitans ve P. gingivalis'in varlığı RT-PCR ile tespit edildi. Bulgular: Mikrobiyolojik analizin sonunda herhangi bir grupta H. pylori tespit edilmedi. Bununla birlikte, agresif periodontit grubunda yüksek oranda A. actinomycetemcomitans (%97.1) ve P. gingivalis (%100) görülmüştür. Bununla birlikte, A. actinomycetemcomitans ve P. gingivalis, kronik periodontitisli hastaların sırasıyla %30 ve %21.7'sinde bulunmuştur. A. actinomycetemcomitans ve P. gingivalis gingivitisli hastaların %24.6'sında bulundu. Sonuç: H. pylori, örneklerde saptanmamış olması, subgingival plağın bu bakteri için birincil rezervuar olmayabileceğini gösterdi
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    Analysing subgingival plaque with regard to H. Pylori at chronic and aggressive periodontitis patients
    (Cumhuriyet University Faculty of Dentistry, 2018) Ustaoglu, Gulbahar; Ercan, Esra; Korkmaz, Meltem Zihni; Uzun, Bilge Cansu; Buruk, Celal Kurtulus
    Background: Helicobacter pylori (H. Pylori) is a Gram (-), microaerophilic bacteria and the etiological factor of chronic active gastritis and peptic ulcer. Some studies indicated that this bacterium found at oral cavity which is a potential reservoir for stomach. Several studies showed that H. pylori may found in saliva and subgingival plaque of chronic periodontitis patients. However, there is no data related to aggressive periodontitis patients. In this study, we aimed to determine the prevalence of H. pylori in subgingival plaque samples of chronic, aggressive periodontitis and gingivitis patients and to increase the awareness of the patients for gastric problems. Materials and Methods: This study included 155 patients (61 with gingivitis, 60 with chronic periodontitis, and 34 with aggressive periodontitis) who did not have gastric disease symptom and did not use antibiotics in the last 3 months. The subgingival plaque samples were taken using sterile paper points. The existence of H. pylori, A. actinomycetemcomitans, and P. gingivalis was detected by RT-PCR. Results: H. pylori was not detected in any groups at the end of microbiological analysis. However, a high occurrence of A. actinomycetemcomitans (97.1%) and P. gingivalis (100%) was observed in the aggressive periodontitis group. However, A. actinomycetemcomitans and P. gingivalis were found in 30% and 21.7% of patients, respectively, with chronic periodontitis. A. actinomycetemcomitans and P. gingivalis were found in 24.6% of patients in the gingivitis group. Conclusions: H. pylori were not detected in samples, indicating that subgingival plaque may not be a primary reservoir for this bacterium. © 2018 Cumhuriyet University Faculty of Dentistry.
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    Comparison of clinical efficacy and patient acceptance of interdental brush and silicone coated interdental pick: a randomized split-mouth, prospective clinical trial
    (Springer Heidelberg, 2020) Ustaoğlu, Gülbahar; Ercan, Esra; Gümüş, Kerem Çağlar
    Objectives The aim of this split-mouth, prospective controlled study was to compare the effects of two different interdental devices on clinical plaque elimination, gingival bleeding and patient acceptance and comfort. Materials and methods Thirty participants who had been diagnosed with gingivitis were included in the study. After professional oral prophylaxis and a 3-day washout period, patients were advised to use two test devices (TePe Interdental Brushes Original and TePe EasyPick (TM), Malmo, Sweden) according to instructions. The plaque index (Turesky modification of the Quigley and Hein Index) and bleeding index (Papillary Bleeding Index) were recorded at baseline and after 2 weeks. Patient satisfaction and comfort were assessed with a questionnaire. Results Both of the tested devices improved the plaque and bleeding index scores. There were no differences between the two sides in terms of time-dependent changes. The patients felt more satisfied with the cleansing capacity and more comfortable with the use of SCIP compared with IDB (p = 0.001). Pain sensation with the use of SCIP was significantly lower than with IDB (p = 0.002). Conclusion The clinical efficiency of the tested interdental devices was similar in terms of removing plaque and decreasing bleeding. However, SCIP were found to be more comfortable and preferable to IDB.
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    Nano-hydroxyapatite airborne-particle abrasion system as an alternative surface treatment method on intraorally contaminated titanium discs
    (Quintessence Publishing Co Inc, 2020) Gümüş, Kerem Çağlar; Ustaoğlu, Gülbahar; Kara, Levent; Ercan, Esra; Albayrak, Önder; Tunalı, Mustafa
    The aim of this study was to test the nano-hydroxyapatite powder decontamination method on intraorally contaminated titanium discs and to compare this method with current decontamination methods in the treatment of peri-implantitis. Contaminated discs were assigned to six treatment groups (n = 10 each): titanium hand curette; ultrasonic scaler with a plastic tip (appropriate for titanium); ultrasonic scaler with a plastic tip (appropriate for titanium) + H2O2; short-term airflow system (nano-hydroxyapatite airborne-particle abrasion for 30 seconds); long-term airflow system (nano-hydroxyapatite airborne-partide abrasion for 120 seconds); Er:YAG laser (120 mJ/pulse at 10 Hz). There were also two control groups (n = 10 each): contaminated disc (negative control) and sterile disc (positive control). Scanning electron microscopy, energy-dispersive x-ray spectroscopy, and dynamic contact angle analysis were used to determine the most effective surface-treatment method. The highest percentage of carbon (C) atoms was observed in the negative control group, and the lowest percentage of C atoms was found in the long-term airflow group, followed by the short-term airflow, laser, ultrasonic + H2O2, ultrasonic, and mechanical groups. When the groups were examined for wettability, the lowest contact angle degree was observed in the long-term airflow, short-term airflow, and laser groups. Nano-hydroxyapatite and laser treatments for detoxifying and improving infected titanium surfaces may show the most suitable results for reosseointegration.