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Öğe Construction of hydrophobic nanoparticles based surface plasmon resonance biosensor for lysozyme detection(Amer Chemical Soc, 2017) Saylan, Yeşeren; Yılmaz, Fatma; Derazshamshir, Ali; Denizli, Adil[No Abstract Available]Öğe Development of surface plasmon resonance sensors based on molecularly imprinted nanofilms for sensitive and selective detection of pesticides(Elsevier Science Sa, 2017) Saylan, Yeşeren; Akgönüllü, Semra; Çimen, Duygu; Derazshamshir, Ali; Bereli, Nilay; Yılmaz, FatmaPesticides have been utilized in agriculture for decades. However, their widespread use has increased multiple concerns due to their known and suspected toxicities on long-term human health risks in scientific and industrial communities. Thus, detecting pesticides will have a great impact on their management, as well as improve their toxicity effects over humans. Here, we fabricate molecularly imprinted nanofilms and integrate them with surface plasmon resonance (SPR) sensors for sensitive, selective, fast and real-time detection of multiple pesticides, including cyanazine (SNZ), simazine (SMZ) and atrazine (ATZ). The molecularly imprinted nanofilms onto the SPR gold surfaces are prepared via UV polymerization reactions, which consist of N-methacryloyl-L-phenylalanine methyl ester (MAPA) as a functional monomer, 1-vinylimidazole (VIM) as a co-monomer, and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. The real-time measurements on SPR sensor provide a detection range from 0.10 to 6.64 nM, as well as denote a limit of detection (LOD) values of 0.095, 0.031 and 0.091 nM for SNZ, SMZ and ATZ, respectively. Furthermore, we perform selectivity test, where SNZ, SMZ and ATZ are examined as competitor agents. Overall, the pesticide imprinted SPR sensors have been found to be highly selective and sensitive. These SPR sensors also hold great potential to be used an alternative method for the existing pesticide monitoring approaches due to their reusability, fast response, and easy-to-use properties, as well as can be tailored to detect and real-time monitor of other pesticides. (C) 2016 Elsevier B.V. All rights reserved.Öğe Dopamine-imprinted monolithic column for capillary electrochromatography(Wiley, 2017) Aşır, Süleyman; Sarı, Duygu; Derazshamshir, Ali; Yılmaz, Fatma; Şarkaya, KorayA dopamine-imprinted monolithic column was prepared and used in capillary electrochromatography as stationary phase for the first time. Dopamine was selectively separated from aqueous solution containing the competitor molecule norepinephrine, which is similar in size and shape to the template molecule. Morphology of the dopamine-imprinted column was observed by scanning electron microscopy. The influence of the organic solvent content of mobile phase, applied pressure and pH of the mobile phase on the recognition of dopamine by the imprinted monolithic column has been evaluated, and the imprinting effect in the dopamine-imprinted monolithic polymer was verified. Developed dopamine-imprinted monolithic column resulted in excellent separation of dopamine from structurally related competitor molecule, norepinephrine. Separation was achieved in a short period of 10 min, with the electrophoretic mobility of 5.81x10(-5)m(2)V(-1)s(-1) at pH 5.0 and 500mbar pressure.Öğe Megaporous poly(hydroxy ethylmethacrylate) based poly(glycidylmethacrylate-N-methacryloly-(L)-tryptophan) embedded composite cryogel(Elsevier Science Bv, 2015) Türkmen, Deniz; Bereli, Nilay; Derazshamshir, Ali; Perçin, Işık; Shaikh, Huma; Yılmaz, FatmaOne-step activation, purification, and stabilization of lipase enzyme were performed by using composite hydrophobic support at low ionic strength with increased surface area during embedding process. A novel hydrophobic poly(hydroxyethylmethacrylate) [PHEMA] based, poly(glycidyl methacrylate-N-methacryloly-(L)-tryptophan) [PGMATrp] bead embedded composite cryogel membrane having specific surface area of 195 m(2)/g was used as hydrophobic matrix for adsorption of commercial Candida Rugosa lipase in a continuous system. PGMATrp embedded PHEMA cryogel membrane with 60-100 mu m pore size was obtained by dispersion polymerization of GMA and MATrp to form PGMATrp beads followed by embedding of PGMATrp to HEMA via APS and TEMED redox pair. The introduction of hydrophobic MATrp monomer into bead structure aiming to increase interaction between lipase and composite membrane was estimated using nitrogen stoichiometry of elemental analysis and found to be 239 mu mol/g of polymer. Hydophobicity increment due to embedding process was confirmed by measuring contact angle, it was found 42 degrees and 48.4 degrees for the PHEMA and PHEMA/PGMATrp composite cryogel respectively. Some parameters i.e. pH, flow-rate, protein concentration, temperature, salt type and ionic intensity were evaluated on the adsorption capacity in a continuous system. Fast protein liquid chromatography (FPLC) studies were performed for specific adsorption of lipase onto the PHEMA/PGMATrp embedded composite cryogel membrane. (C) 2015 Elsevier B.V. All rights reserved.Öğe Molecular imprinting of macromolecules for sensor applications(Mdpi, 2017) Saylan, Yeşeren; Yılmaz, Fatma; Özgür, Erdoğan; Derazshamshir, Ali; Yavuz, HandanMolecular recognition has an important role in numerous living systems. One of the most important molecular recognition methods is molecular imprinting, which allows host compounds to recognize and detect several molecules rapidly, sensitively and selectively. Compared to natural systems, molecular imprinting methods have some important features such as low cost, robustness, high recognition ability and long term durability which allows molecularly imprinted polymers to be used in various biotechnological applications, such as chromatography, drug delivery, nanotechnology, and sensor technology. Sensors are important tools because of their ability to figure out a potentially large number of analytical difficulties in various areas with different macromolecular targets. Proteins, enzymes, nucleic acids, antibodies, viruses and cells are defined as macromolecules that have wide range of functions are very important. Thus, macromolecules detection has gained great attention in concerning the improvement in most of the studies. The applications of macromolecule imprinted sensors will have a spacious exploration according to the low cost, high specificity and stability. In this review, macromolecules for molecularly imprinted sensor applications are structured according to the definition of molecular imprinting methods, developments in macromolecular imprinting methods, macromolecular imprinted sensors, and conclusions and future perspectives. This chapter follows the latter strategies and focuses on the applications of macromolecular imprinted sensors. This allows discussion on how sensor strategy is brought to solve the macromolecules imprinting.Öğe Molecularly imprinted hydrophobic polymers as a tool for separation in capillary electrochromatography(Royal Soc Chemistry, 2015) Derazshamshir, Ali; Yılmaz, Fatma; Denizli, AdilThe use of molecular imprinted polymers (MIPs), which provides a means for preparing stationary phases with predetermined selectivity for a target molecule in capillary electrochromatography (CEC), is attractive because it combines selectivity, higher separation efficiency and shorter analysis time. A bisphenol A (BPA)-imprinted monolithic capillary BPA/PMAPA column was synthesized for the selective separation of bisphenol A (BPA) from aqueous solutions containing the competitor molecule phenol (PH), which is similar in size and shape to the template molecule. BPA-imprinted monolithic column was prepared in the presence of the template molecule, BPA, which results in the formation of recognition cavities complementary to the template molecule, after the removal of template molecule. An amino acid based monomer, N-methacryloyl-L-phenyl alanine (MAPA), was used as the functional monomer. The new stationary phase contains both charged and hydrophobic groups originating from MAPA monomer, which behaves as both an electroosmotic flow (EOF) supplier and a hydrophobic matrix. The MAPA containing BPA imprinted column behaves as a mixed mode stationary phase, as ion exchanger and hydrophobic matrix depending on the pH of the medium. Scanning electron microscopy was used to identify the structural features of the molecular imprinted column. MIP column performance was evaluated by the electrochromatographic separation of alkylbenzenes. The novelty of this work originated from dual separation mechanism shown by MAPA, which has the ability to form both hydrophobic and electrostatic interactions by the charged and hydrophobic groups of phenylalanine amino acid. This new column with mixed-mode characteristics was then used successfully as the stationary phase in CEC for the selective separation of BPA in MIP systems.Öğe Phenol removal from wastewater by surface imprinted bacterial cellulose nanofibres(Taylor & Francis Ltd, 2020) Derazshamshir, Ali; Göktürk, Ilgım; Tamahkar, Emel; Yılmaz, Fatma; Sağlam, Necdet; Denizli, AdilIn this study, we have reported a novel wastewater treatment technique by phenol imprinted bacterial cellulose (BC-MIP) nanofibres with high specificity and adsorption capacity. N-methacryloyl-(L) phenylalanine methyl ester (MAPA) functional monomer was used to create specific binding sites for the template molecule phenol via electrostatic and hydrophobic interactions. BC-MIP nanofibres were synthesized by surface imprinting approach in the presence of different amounts of total monomer (% weight), monomer/template ratio and polymerization time. Then, the nanofibres were characterized by FTIR-ATR, surface area analysis (BET), elemental analysis, scanning electron microscopy (SEM) and contact angle measurements. Adsorption studies were performed with respect to pH, temperature and ionic strength, and the adsorption capacity was calculated by using the spectrophotometer. In order to desorb the adsorbed phenol from BC-MIP nanofibres, 0.1 M NaCl solution was used. Besides, BC-MIP nanofibres were applied to real wastewater samples from Ergene basin in Turkey. The suitable equilibrium isotherm was determined as Langmuir isotherm. To evaluate the selectivity of the BC-MIP nanofibres, similar molecules were utilized as competitor molecules, which were 2-chlorophenol, 4-chlorophenol and 2,4-dichlorophenol. Electrostatic interactions were found to contribute to the generation of specific recognition binding sites. The results have shown that imprinting of phenol was achieved successfully with high adsorption capacity. The phenol removal efficiency was reported up to 97%. BC-MIP nanofibres were used 10 times with a negligible decrease in adsorption capacity. [GRAPHICS] .Öğe Poly(vinyl alcohol)/polyethyleneimine (PVA/PEI) blended monolithic cryogel columns for the depletion of haemoglobin from human blood(Taylor & Francis Inc, 2016) Gokturk, Ilgim; Derazshamshir, Ali; Yilmaz, Fatma; Denizli, AdilWe have synthesized PVA/PEI monolithic cryogel columns chelated with Cu2+ ions as a model adsorbent, which is capable of binding haemoglobin (Hb) from human blood. The goal of this study is to perform the depletion of Hb via a single and easy process to be useful in proteomic studies. PVA/PEI-Cu2+ cryogel columns were subjected to adsorption studies of Hb from both aqueous solution and human plasma to evaluate the extent of interaction between cryogel columns and Hb. The effects of experimental parameters, such as pH, Hb equilibrium concentration, adsorption time, temperature, and ionic strength, on Hb adsorption capacity were investigated.Öğe Polymethacryloyl-L-Phenylalanine [PMAPA]-based monolithic column for capillary electrochromatography(Oxford Univ Press Inc, 2019) Derazshamshir, Ali; Aşır, Süleyman; Göktürk, Ilgım; Ektirici, Sisem; Yılmaz, Fatma; Denizli, AdilThe ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 x 10(-6) m(2) V-1 s(-1) and 7.60 x 10(-6) m(2) V-1 s(-1) for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.Öğe Potentialities of nanosilver-based thin film coatings for medical device and implants(Elsevier, 2024) Göktürk, Ilgım; Guler, Kıvılcım Caktu; Derazshamshir, Ali; Yılmaz, Fatma; Denizli, AdilThe physicochemical properties of a biomaterial surface, together with the antifouling property of the device, that is, its long-term antibacterial performance, are crucial for developing new coating technologies without toxic effects on mammalian cells and without causing drug resistance. Nanotechnology-based strategies hold promise for preventing drug-resistant biofilm infections of biomaterials and medical devices. One of the best methods for minimizing hospital-acquired infections is to use biocompatible antimicrobial polymers whose surfaces are modified by biocidal substances, such as antimicrobial peptides, antibiotics, and silver nanoparticles. Nanosilver is the most effective metal with superior antimicrobial properties, because of its oligodynamic effect, versatile biocidal mechanisms of action, and low cytotoxicity for humans. Comprehensive information on various surface modification methods for biomaterial coatings that can be utilized to fix silver nanoparticles on polymer-based composites of both synthetic and natural origin, which are candidates for medical devices and implants was provided in this chapter. Finally, the issues about safety concerns, potential toxicity, and compatibility of AgNPs-polymeric nanocomposites used in biomedical devices and implants were discussed. © 2024 Elsevier Ltd. All rights reserved.Öğe Preparation of cryogel columns for depletion of hemoglobin from human blood(Taylor & Francis Ltd, 2016) Derazshamshir, Ali; Baydemir, Gözde; Yılmaz, Fatma; Bereli, Nilay; Denizli, AdilIn this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.Öğe Rapid sensing of Cu+2 in water and biological samples by sensitive molecularly imprinted based plasmonic biosensor(Elsevier, 2019) Safran, Volkan; Gokturk, Ilgrm; Derazshamshir, Ali; Yilmaz, Fatrna; Saglam, Necdet; Denizli, AdilIn this study, copper (II) ion (Cu+2) imprinted poly(hydroxyethyl methacrylate-N-metacryloyl-(L)-cysteine methyl ester [PHEMAC-Cu+2] nanoparticles were synthesized by two-phase mini-emulsion polymerization method and applied to the SPR sensor chip surface for the selective determination of the Cu+2 ions in both aqueous solution, Cu+2-spiked artificial urine and physiological serum samples to investigate the effects of metabolite residues during the analysis. The non imprinted [PHEMAC] nanoparticles were synthesized by applying the same procedure for the [PHEMAC-Cu+2] nanoparticle synthesis except the addition of Cu+2 ions as a control experiment to evaluate the selectivity of the [PHEMAC-Cu+2] nanoparticles. Roughness differences between [PHEMAC-Cu+2] and [PHEMAC] nanoparticles showed that the imprinting process of Cu+2 ions was performed successfully. [PHEMAC-Cu+2] and [PHEMAC] SPR biosensors prepared by attaching nanoparticles onto the surface of sensor chips, were characterized by atomic force microscope, ellipsometer, contact angle measurements. Langmuir adsorption model was found the most applicable model for this affinity system. Results showed that Cu+2 affinity regions on the surface of [PHEMAC-Cu+2] SPR biosensor were homogeneously distributed and have a monolayer structure. Having the high imprinting efficiency with the imprinting factor of 4.74, the [PHEMAC-Cu+2] SPR biosensor was found to show more selectivity towards the target Cu+2 than the non-imprinted [PHEMAC] SPR biosensor. The selectivity studies of [PHEMAC-Cu+2] SPR biosensors for Cu+2 detection were investigated by using Zn+2 and Ni+2 solutions selected as competitor molecules. The results of intraday and interday precision studies were carried out to ascertain the reproducibility of the proposed method and reported as percent relative standard deviation (%RSD) value.Öğe S-citalopram imprinted monolithic columns for capillary electrochromatography enantioseparations(WILEY, 2021) Derazshamshir, Ali; Göktürk, Ilgım; Yılmaz, Fatma; Denizli, AdilIn this study, the molecular imprinting method was used to separate enantiomeric forms of chiral antidepressant drug, R,S-citalopram (R,S-CIT) in aqueous solution by CEC system combining the advantages of capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). For that, an amino acid-based molecularly imprinted monolithic capillary column was designed and used as a stationary phase for selective separation of S-citalopram (S-CIT) for the first time. S-CIT was selectively separated from the aqueous solution containing the other enantiomeric form of R-CIT, which is the same in size and shape as the template molecule. Morphology of the molecularly imprinted (MIP S-CIT) and non-imprinted (NIP S-CIT) monolithic capillary columns was observed by scanning electron microscopy. Imprinting efficiency of MIP S-CIT monolithic capillary column used for selective S-CIT separation was verified by comparing with NIP S-CIT and calculated imprinting factor (I.F:1.81) proved the high selectivity of the MIP S-CIT for S-CIT. Cavities formed for S-CIT form enabled selective (alpha = 2.08) separation of the target molecule from the other enantiomeric R-CIT form. Separation was achieved in a short period of 10 min, with the electrophoretic mobility of 7.68 x 10(-6) m(2)/Vs for R,S-CIT at pH 7.0 10 mM PB and 50% ACN ratio. The performance of both MIP S-CIT and NIP S-CIT columns was estimated by repeating the R,S-CIT separations with intra-batch and inter-batch studies for reproducibility of retention times of R,S-CITs. Estimated RSD values that are lower than 2% suggest that the monolithic columns separate R,S-CIT enantiomers without losing separation efficiency.Öğe Separation of D,L-Ampicillin by ligand exchange-micellar electrokinetic chromatography(Biointerface Research Applied Chemistry, 2019) Sarı, Duygu; Derazshamshir, Ali; Aşır, Süleyman; Göktürk, Ilgım; Yılmaz, Fatma; Denizli, AdilIn this study, D,L-ampicillin separation was carried out by ligand exchange-micellar electrokinetic chromatography method using L-Lysine monohydrochloride as a ligand and copper (II) sulfate pentahydrate is a central ion supplier. Isomeric separations were performed using capillary electrophoresis (CE) instrument, in which SDS-L-Lys-Cu+2 micelle complex was used as a pseudostationary phase. The effect of pH, SDS amount, applied electrical field, pressure, organic solvent ratio and ampicillin D,L-ratios were investigated. Fast,inexpensive and sensitive approach for the simultaneous separation of D,L-ampicillin in both aqueous and real antibiotic sample was performed using CE coupled with UV detector. The separation was achieved in a short period of 7 minutes with high-sensitivity andlow-detection limit of 1.25 mu M by the developed SDS-L-Lys-Cu+2 micelle-chiral selector complexes without using any extra process such as imprinting or spacer arms for the immobilization of the ligands.Öğe Surface plasmon resonance based nanosensors for detection of triazinic pesticides in agricultural foods(Elsevier, 2017) Yılmaz, Fatma; Saylan, Yeşeren; Akgönüllü, Semra; Çimen, Duygu; Derazshamshir, Ali; Bereli, Nilay; Denizli, AdilHerein, we have focused on the preparation of triazinic pesticide imprinted SPR nanosensors for detection of herbicides. Triazinic pesticides are weedkillers that are related with possible carcinogenic effects, birth defects, and menstrual problems when uptake by humans. Although there are restrictions and bans on their use in some countries they are still one of the most widely used pesticides in the world. The development of rapid, sensitive, and inexpensive diagnosis tools for environmental and biological monitoring is currently a research area of great interest. Surface plasmon resonance (SPR) nanosensors have been used widely for the detection of triazinic pesticides because of their simplicity, lack of requirement for labeling and ease of miniaturization, low cost, high specificity and sensitivity, and real-time measurement. Molecularly imprinted polymers that have molecular recognition talent, are easy to prepare, less expensive, stable, and can be manufactured with good reproducibility, are used for the creation of biorecognitive surfaces on the SPR nanosensors. Herein, we have focused on the production of triazinic pesticide-imprinted SPR nanosensors. © 2017 Elsevier Inc. All rights reserved.Öğe Surface plasmon resonance sensors for medical diagnosis(Springer Berlin Heidelberg, 2018) Saylan, Yeşeren; Yılmaz, Fatma; Özgür, Erdogan; Derazshamshir, Ali; Bereli, Nilay; Yavuz, Handan; Denizli, AdilSurface plasmon resonance (SPR) sensors have fascinated impressive attention to detect clinically related analytes in recent years. SPR sensors have also multiple advantages over existing conventional diagnostic tools such as easy preparation, no requirement of labeling, and high specificity and sensitivity with low cost, and they provide real-time detection capability. There are some articles and reviews in literature focusing on the applications of SPR-based sensors for the diagnosis of medically important entities such as proteins, cells, viruses, disease biomarkers, etc. These articles generally give information on the determination of such structures merely, whereas this presented manuscript combines recent literature for most of the medically important structures together including proteins, hormones, nucleic acids, whole cells, and drugs that especially the latest applications of SPR sensors for medical diagnosis to follow up new strategies and discuss how SPR strategy is brought to solve the medical problems.Öğe Surface plasmon resonance sensors for real-time detection of cyclic citrullinated peptide antibodies(Taylor & Francis Inc, 2016) Dibekkaya, Hüseyin; Saylan, Yeşeren; Yılmaz, Fatma; Derazshamshir, Ali; Denizli, AdilSurface plasmon resonance (SPR) sensors have been used for detection of various biomolecules because of their simplicity, high specificity and sensitivity, real-time detection, low cost, and no requirement of labeling. Recently, molecularly imprinted polymers that are easy to prepare, less expensive, stable, have talent for molecular recognition and also are used for creation selective binding sites for target molecule on the SPR sensors. Here, we show that preparation of cyclic citrullinated peptide antibody (anti-CCP) imprinted SPR sensor to detect CCP antibodies. For this purpose, anti-CCP/AAm pre-complex was synthesized by interacting acrylamide (AAm) monomer with anti-CCP. Then, anti-CCP imprinted (anti-CCP/PAAm) SPR sensor was obtained by reacting with anti-CCP/AAm pre-complex in the presence of the crosslinker, and initiator/activator pair. Besides this, non-imprinted (PAAm) SPR sensor was also prepared without using anti-CCP template. The SPR sensors were characterized and then adsorption-desorption studies were performed with pH 7.0 phosphate buffer (10mM) and acetic acid (10%) with Tween 20 (1%) in pH 7.0 phosphate buffer. Selectivitiy of sensors was investigated by using immunoglobulin M (IgM) and bovine serum albumin (BSA). To determine the adsorption model of interactions between anti-CCP solutions and anti-CCP/PAAm SPR sensor, different adsorption models were performed. The calculated maximum reflection, detection limit, association and dissociation constants were 1.079RU/mL, 0.177RU/mL, 0.589RU/mL and 1.697mL/RU, respectively. Repeatability experiments of anti-CCP/PAAm SPR sensor was performed four times with adsorption-desorption-regeneration cycles without any performance losing. Results showed that anti-CCP/PAAm SPR sensor had high selectivity and sensitivity for detection of CCP antibodies.Öğe Synthesis of hydrophobic nanoparticles for real-time lysozyme detection using surface plasmon resonance sensor(Wiley, 2017) Saylan, Yeşeren; Yılmaz, Fatma; Derazshamshir, Ali; Yılmaz, Erkut; Denizli, AdilDiagnostic biomarkers such as proteins and enzymes are generally hard to detect because of the low abundance in biological fluids. To solve this problem, the advantages of surface plasmon resonance (SPR) and nanomaterial technologies have been combined. The SPR sensors are easy to prepare, no requirement of labelling and can be detected in real time. In addition, they have high specificity and sensitivity with low cost. The nanomaterials have also crucial functions such as efficiency improvement, selectivity, and sensitivity of the detection systems. In this report, an SPR-based sensor is developed to detect lysozyme with hydrophobic poly (N-methacryloyl-(L)-phenylalanine) (PMAPA) nanoparticles. The SPR sensor was first characterized by attenuated total reflection-Fourier transform infrared, atomic force microscope, and water contact angle measurements and performed with aqueous lysozyme solutions. Various concentrations of lysozyme solution were used to calculate kinetic and affinity coefficients. The equilibrium and adsorption isotherm models of interactions between lysozyme solutions and SPR sensor were determined and the maximum reflection, association, and dissociation constants were calculated by Langmuir model as 4.87, 0.019nM(-1), and 54nM, respectively. The selectivity studies of SPR sensor were investigated with competitive agents, hemoglobin, and myoglobin. Also, the SPR sensor was used four times in adsorption/desorption/recovery cycles and results showed that, the combination of optical SPR sensor with hydrophobic ionizable PMAPA nanoparticles in one mode enabled the detection of lysozyme molecule with high accuracy, good sensivity, real-time, label-free, and a low-detection limit of 0.66nM from lysozyme solutions. Lysozyme detection in a real sample was performed by using chicken egg white to evaluate interfering molecules present in the medium.Öğe Triazine herbicide imprinted monolithic column for capillary electrochromatography(Wiley, 2015) Aşır, Süleyman; Derazshamshir, Ali; Yılmaz, Fatma; Denizli, AdilTrietazine was selectively separated from aqueous solution containing the competitor molecule cyanazine, which is similar in size and shape to the template molecule. Structural features of the molecularly imprinted column were figured out by SEM. The influence of the mobile-phase composition, applied electrical field, and pH of the mobile phase on the recognition of trietazine by the imprinted monolithic polymer has been evaluated, and the imprint effect in the trietazine-imprinted monolithic polymer was demonstrated by an imprinting factor. The optimized monolithic column resulted in separation of trietazine from a structurally related competitor molecule, cyanazine. In addition, fast separation was obtained within 6 min by applying higher electrical field, with the electrophoretic mobility of 2.97 x 10(-8) m(2)V(-1)s(-1) at pH 11.0.
