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    Alleviation of Cadmium Stress on Pollens of Quince Varieties Through Epibrassinolide
    (Springer, 2024) Muradoglu, Ferhad; Beyhan, Omer; Demir, Taki; Manzoor, Muhammad Arsalan; Sonmez, Ferit; Balik, Hueseyin Irfan
    Environmental factors significantly impact plant physiological processes, especially during pollination and fertilization. Pollen germination (PG) and pollen tube length (PTL), crucial in plant reproduction, are particularly vulnerable to environmental contaminants that affect fruit sets adversely. This study investigated the impact of varying cadmium (Cd) doses, a significant toxic heavy metal, and the alleviative potential of external 24-epibrassinolide (24-epiBL) applications on cadmium stress, determined through PG and PTL. Quince pollen viability rates, assessed using the TTC (2-3-5-triphenyl tetrazolium chloride) viability test, varied between 38.58% and 55.80%. PG and PTL rates decreased in response to higher Cd doses. The application of epiBL increased PG and PTL, but a notable decline was observed at the 2.00 mu M application. A positive relationship between the application dosage of epiBL and its alleviation effect on Cd stress on PG and PTL was observed except for the 2.00 mM. Results revealed variability in PG and PTL rates among quince varieties, and PG and PTL rates decreased with higher Cd doses. This study suggests that epiBL up to 0.50 mM is an efficient tool for alleviating Cd stress in quince pollens.
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    Effects of some rhizobacteria and indole-3-butyric acid on rooting of black and white mulberry hardwood cuttings
    (Pakistan Agricultural Scientists Forum, 2018) Zenginbal, Hamdi; Demir, Taki
    This research was conducted to determine the effects of treatments with indole-3-butric acid (0, 2, 4 and 6 g l(-1) IBA) and plant growth promoting rhizobacteria (PGPR) (Burkholderia gladii-BA7, Bacillussubtilus-OSU142 and Bacillusmegatorium-M3) strains have on rooting and root growth of hardwood stem cuttings of black and white mulberry both alone and in combination with each bacterial strain. Cuttings in the control group were treated with 50% ethanol + 50% distilled water. The hardwood stem cuttings (middle parts of one-year-old shoots) for rooting were selected from 15-year-old healthy donor black and white mulberries trees on February 1, 2015 and 2016 in Bolu, Turkey. The treated cuttings were placed in perlite medium in unheated trays of a greenhouse with automated misting system for 90 days. The rooting rate, root number, root length and diameter were evaluated. The rooting rate varied from 12.0 to 85.0%. The number of root varied from 2.05 to 10.19. The root length varied from 2.75 to 8.72 cm. The root diameter varied from 0.81 to 2.49 mm. The results indicated that treatment with 4 g l(-1) IBA plus B. megatorium-M3 solution had a profound effect in increasing rooting capacity and quality in comparison to the control, and all other PGPR and IBA treatments. Overall, rooting rate and root quality of black mulberry hardwood cuttings was found to be lower than those of white mulberry. Moreover, 4 g l(-1) IBA was the most appropriate dose and B. megatorium-M3 bacterial strain was the most appropriate rhizobacteria for rooting of mulberry cuttings.
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    The grafting success of fourteen genotypes grafted on three different rootstocks on production of sweet cherry (Prunus avium L.) sapling
    (Wydawnictwo Akad Rolniczej W Lublinie, 2017) Zenginbal, Hamdi; Demir, Taki; Demirsoy, Hüsnü; Beyhan, Ömer
    This study was conducted in Canakkale Province, Bayramic County between 2011 and 2013. The aim of this study was to determine the best rootstock x genotype combination by grafting 14 standard and local sweet cherry genotypes cultivated in Amasya Province on Mazzard, Gisela 5 and Gisela 6 rootstocks with T-budding method. Graftings were conducted on August 15th in both years. Following the grafting, the average daily temperature and relative humidity values in addition to bud sprout ratios and graft shoot lengths and diameters were examined. The experiment location was suitable for fall T-budding based on obtained climate data. The results of research showed that cherry genotypes and rootstocks had significant effects on bud sprout ratio. The best bud sprout rates among the sweet cherry genotypes and rootstocks were obtained from 'Kargayuregi' genotype (100%) and Gisela 6 rootstock (91.4 and 94.5%, respectively) in both year. Graft shoot lengths and diameters showed differences depending on genotypes and rootstocks. Among the sweet cherry genotypes and rootstocks, the best graft shoot length were obtained from 'Kargayuregi' genotype and Gisela 5 rootstock in both year. The highest graft shoot diameter values among the sweet cherry genotypes and rootstocks were obtained from 'Bing' genotype and Gisela 5 rootstock in both years. As a result, it was shown that the 14 sweet cherry genotypes cultivated in Amasya can be successfully grafted on Mazzard, Gisela 5 and Gisela 6 cherry rootstocks and that 1(st) and 2(nd) class sweet cherry saplings can be produced.