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Öğe Effects of an extract of Salvia Miltiorrhiza on a penicillin-induced epilepsy model in rats(Springer, 2015) Bahadır, Anzel; Demir, Şerif; Orallar, Hayriye; Beyazçiçek, Ersin; Öner, FerideIn a penciling-induced epilepsy model, Wistar rats (16 males, 16 females) were i.p. administered with an extract of Salvia miltiorrhiza (SmE; total dose 50 mg/kg) once a day for 15 days. The rats were divided into four equal groups, control and SmE-treated for each sex. After the treatment period, an epilepsy model was produced by penicillin G injection (500 IU) into the motor cortex; the electrocorticogram (EcoG) was recorded for 120 min, and statistical analysis was performed. In the male control group with penicillin-induced epilepsy, the spike frequency was significantly (P < 0.05) higher than that in the female control group. The frequency values have been significantly (P < 0.01) increased within the observation period in the female SmE-treated group, while the respective values significantly (P < 0.05) decreased in the analogous male group. There were insignificant differences in the amplitude values and latency to onset of the spike/wave events between female/male SmE and female/male control groups (P > 0.05). Thus, the SmE exerts anticonvulsant effects in the male rat group, while its effect should be characterized as proconvulsant in the female group in the penicillin-induced epilepsy model. The difference (related to the presence of estrogen analogs in the SmE) is determined by dissimilar hormonal backgrounds in males and females. The SmE may be considered as the base for development of anticonvulsant drugs for clinical therapy of epilepsy in the future.Öğe The effects of nigella sativa extract and chronic exercise application on penicillin-induced epilepsy model in Mongolian gerbils(Wiley-Blackwell, 2016) Çetinkaya, Ayhan; Demir, Şerif; Orallar, Hayriye; Kayacan, Yıldırım; Beyazçiçek, ErsinAim: To evaluate the role of treadmill exercise on the oxidative stress in Mongolian gerbils with penicillin-induced epilepsy. Methods: This experimental study included 18 male Mongolian gerbils which were divided into three groups; sham-control group, penicillin group (500 units) and exercise + penicillin (500 units) group. Each animal group was composed of six Mongolian gerbils. The epileptiform activity was verified by electrocorticographic recordings. Results: The latency of the penicillin+exercise group was longer than the penicillin group, but this difference was not statistically significant. Following the penicillin administration, spike wave frequencies of epileptiform activity in the 10, 30, and 35 minutes were significantly lower in the penicillin+exercise group, compared with the penicillin group. There were generally significant decreases in the spike wave amplitude medians in the penicillin+exercise groups compared with the penicillin group in all time periods between 0 and 5 minutes. The serum superoxide dismutase, catalase and glutathione peroxidase levels increased in the penicillin+exercise group compared with those in the penicillin group. Conclusion: The results of present study indicate that regular exercise may contribute to the amelioration of epileptic activity by increasing the antioxidant effect. Keywords: Penicillin-induced epilepsy; treadmill exercise; oxidative stress; Mongolian gerbilsÖğe Effects of the ATP-dependent K (+)-channel effectors pinacidil and glibenclamide on liver tissue in an experimental model of epilepsy: A histopathological study(2022) Düzcü, Selma Erdoğan; Çetinkaya, Ayhan; Orallar, Hayriye; Demir, ŞerifAim: It is known that most of the antiepileptic drugs have negative effects on the liver. Pinacidil is a\rnonselective opener of KATP channels, including the plasma membrane and mitochondria. Glibenclamide is\ran ATP -dependent K channel blocker ensuring the intake of calcium. Our aim in this experimental study was\rto examine the effects of pinacidil and glibenclamide on the liver tissue of rats with focal epilepsy.\rMethod: Sixty male Sprague Dawley rats (2-4 months old, 200-250 gr) were used in the study. The rats were\rdivided into 4 groups, 15 in each group. The groups were divided into control group, penicillin group, penicillin\r+ pinacidil group and penicillin + glibenclamide group. The craniums of the rats in the control group were\ropened and normal saline was given; Penicillin (2 ?l 500 IU) was intracortically administered to other groups\rand an experimental epilepsy model was created. At the end of the study, liver tissue of rats was taken and\revaluated in terms of vacuolar degeneration, lymphocyte infiltration, vascular congestion, sinusoidal\rdilatation, necrosis, and Kupffer cell proliferation, radial alignment of hepatic cords, central vein and portal\rvein dilatation in hepatocytes.\rResults: Venous congestion, cytoplasmic vacuolization, Kupffer cell proliferation, portal vein dilatation and\rnecrosis were distinct in the group to which pinacidil was administered, and distortion was present in the radial\rsequence (p<0.001). In addition, inflammation, venous congestion and hepatocyte necrosis were found to be\rlower in the glibenclamide given group compared to the control group (p<0.001).\rConclusion: It can be suggested that pinacidil treatment caused negative results in liver histopathological\rparameters, whereas glibenclamide was more protective by reducing inflammation, venous congestion and\rhepatocyte necrosis.Öğe Evaluation of the protective effect of the cup therapy on the epileptic seizure in rats(Düzce Univ, Fac Medicine, 2021) Çetinkaya, Ayhan; Karamaden, Esra Fidan; Göksu, Selim; Bozat, Bihter Gökçe; Demir, ŞerifObjective: Cup therapy has an important place in traditional and complementary medicine applications. The purpose of our study, this is the first time to investigate the protective effect of cup therapy in rats on experimentally generated epileptic seizures in new rat modeling created by different anatomic regions. Methods: A total of 42 Wistar albino rats were randomly divided into 6 groups (n:7). The "new dry cup" was applied to the G1 group, and the "new wet cup" model was applied to the G2. In the G3 group, an "epilepsy model" (PTZ, 35 mg / kg) was created and "diazepam" (2.5 mg / kg) was given to G4. "Dry cup" and "wet cup" models were applied to the G5 and G6 groups, respectively. 24 hours after the cupping therapies, the rats were injected with PTZ and the epilepsy behavior scores of the rats in all groups were recorded for 20-30 minutes. Results: In the 'Open Area' and 'Elevated Plus Maze' tests, there was no behavioral difference between the cup therapy group and the control group (p > 0.05). Given all the parameters, the G4 group significantly reduces the seizure compared to other groups (p < 0.05). There is a significant difference in G2, G5 and G6 groups compared to G3 in the phases parameter (p < 0.05). Conclusions: In this study, the new wet cup therapy (G2), which was applied for the first time, had a protective effect on seizures. G2, G5 and G6 groups are observed to suppress seizures compared to G3. Our findings are expected to contribute greatly to animal model analysis in the future.Öğe Gender Specificity of Genistein Treatment in Penicillin-Induced Epileptiform Activity in Rats(Springer, 2016) Bahadır, Anzel; Demir, Şerif; Orallar, Hayriye; Beyazçicek, Ersin; Çetinkaya, AyhanWe investigated gender-dependent differences of genistein (isoflavone phytoestrogen) treatment in a penicillin-induced experimental epilepsy rat model. Twenty-eight adult Wistar Albino rats (14 females and 14 males) were devided into four groups, control and genistein-treatmed males and females. Genistein (100 mu g/kg, i.p) or saline was given during 15 days before the electrocorticography (ECoG) recordings. The epileptiform activity was induced by penicillin G potassium salt (500 IU) injections into the left somatomotor cortex. Significant differences among the groups were found in the latency to onset of epileptiform activity. This value in the female control group was significantly longer than the latencies in the male control, male genistein, and female genistein groups (respectively, P = 0.002, 0.015, and 0.032). There were no significant differences regarding the spike/wave frequencies and amplitudes in epileptiform activity between female/male genistein and control groups within all observation intervals (P > 0.05). Thus, genistein exerts a proconvulsant effect in the penicillin-induced epilepsy model, and the effect demonstrates the clear gender specificity related to the specificity of hormonal backgrounds in males and females.Öğe Protective effect of cup therapy model in rats on epileptic seizures by determination of different anatomic region(Wiley, 2019) Çetinkaya, Ayhan; Fidan, Esra; Göksu, Selim; Bozat, Bihter Gökçe; Demir, Şerif[No Abstract Available]Öğe Therapeutic effects of carvacrol on beta-amyloid-induced hippocampal neurotoxicity, oxidative stress, and memory impairment in a rat model of alzheimer's disease(Wiley, 2022) Topkara, Kübra Çelik; Kılınç, Erkan; Çetinkaya, Ayhan; Şaylan, Aslıhan; Demir, ŞerifMeeting AbstractÖğe Therapeutic effects of carvacrol on beta-amyloid-induced impairments in in vitro and in vivo models of Alzheimer's disease(WILEY, 2022) Topkara, Kübra Çelik; Kılınç, Erkan; Şaylan, Aslıhan; Demir, Şerif; Çetinkaya, AyhanDue to the complex nature of Alzheimer's disease (AD), it is important to investigate agents with multiple effects in the treatment of AD. Carvacrol possesses anti-acetylcholinesterase, anti-oxidant, and neuroprotective properties. We therefore investigated therapeutic effects of carvacrol on cell viability, oxidative stress, and cognitive impairment in A beta 1-42-induced in vitro and in vivo models of AD. SH-SY5Y cells differentiated into neurons by retinoic acid were pretreated with carvacrol or galantamine before A beta 1-42 administration. For in vivo experiments, a rat model of AD was established by bilateral intrahippocampal injection of A beta 1-42. The groups received 1% DMSO, carvacrol, or galantamine intraperitoneally twice a day (morning and afternoon) for 6 days. Cell viability was determined using MTT and LDH tests. Learning and memory functions were assessed using a passive-avoidance test. Oxidant-antioxidant parameters (MDA, H2O2, SOD, and CAT) and Tau, A beta 1-40, and A beta 1-42 peptide levels in in vitro supernatant or in vivo serum and hippocampal samples were measured using ELISA. Carvacrol increased cell viability and exhibited a protective effect against oxidative stress by preventing A beta 1-42-induced cytotoxicity, LDH release, and increments in MDA and H2O2 levels in vitro. Additionally, it improved memory impairment by reversing A beta 1-42-induced changes on passive-avoidance test. Carvacrol ameliorated A beta 1-42-induced increments in MDA and H2O2 levels in in vitro supernatant and in vivo hippocampal samples. However, none of the treatments changed in vitro SOD and Tau-peptide levels, or in vivo serum levels of MDA, H2O2, SOD, CAT, Tau peptide, A beta 1-40, or A beta 1-42. Our results suggest that multi-target pharmacological agent carvacrol may be promising in treatment of AD by preventing beta-amyloid-induced neurotoxicity, oxidative stress, and memory deficits.
