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    Decoding genetic diversity and population structure of Brassica species by inter primer binding site (iPBS) retrotransposon markers
    (Springer, 2024) Sameeullah, Muhammad; Kayacetin, Fatma; Khavar, Khalid Mahmood; Perkasa, Achmad Yozar; Maesaroh, Siti; Waheed, Mohammad Tahir; Ciftci, Vahdettin
    Marker-assisted breeding serves as a potent tool for screening target germplasm, assessing genetic diversity, and determining breeding potential of a crop. Therefore, inter primer binding site (iPBS)-retrotransposons marker system was employed to evaluate a collection of 33 Brassica genotypes, including 10 Brassica juncea, 5 B. oleracea, 7 Sinapis alba, 5 B. nigra, and 6 B. rapa, were utilized to evaluate their genetic diversity and variations 10 polymorphic primers that generated a total of 144 bands. Various diversity indices were calculated in the studied germplasm, including polymorphism information content (0.13-0.30), effective number of alleles (1.217-1.689), Shannon's information index (0.244-0.531), and gene diversity (0.148-0.370). These indices collectively affirmed substantial genetic variations within the germplasm. Molecular variance analysis revealed that the majority (62%) of genetic variations were present within populations. The Brassica accessions were categorized into three populations utilizing a model-based structure algorithm. Evaluation of diversity indices based on the structure indicated that populations III and II exhibited higher diversity. Principal coordinate analysis and neighbor-joining analysis further corroborated the three distinct populations, confirming the reliability of the STRUCTURE analysis. Notably, the genetic distance assessment identified BN1 and BN3 from B. nigra species and the genotypes BO1 and BO3 from B. oleracea as genetically diverse mustard accessions. The extensive genetic diversity observed within the Brassica germplasm underscores its significance as a valuable genetic resource for comprehensive Brassica breeding programs. Moreover, these accessions hold promise as suitable candidates for heterosis breeding initiatives aimed at improving mustard production.
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    Determination of Some Seed Characteristics of False Flax (Camelina sativa L. Crantz) Genotypes Grown Under Semi-Temperate Conditions
    (Univ Philippines Los Banos, 2022) Subasi, Ilhan; Arslan, Yusuf; Eryigit, Tamer; Ciftci, Vahdettin; Camlica, Mahmut
    Camelina is an alternative low-input oil plant grown in marginal areas in Turkey. This study aimed to determine some quality characteristics of 39 camellia genotypes in ecological conditions in Bolu, Turkey. The field experiments were carried out in an augmented design with randomized blocks. In this work, some important properties such as thousand seed weight, oil content, fatty acid composition, and protein ratio were investigated. Results showed that mean data for thousand seed weight, oil ratio, and protein ratio were 0.63 - 1.5 g, 26.69 - 39.17%, and 21.81 - 29.81%, respectively. Considering the oil content of the genotypes used in the study, the PI 650158 genotype had the highest oil ratio, while the PI 650164 genotype had the highest seed protein ratio. In conclusion, the PI 311735 and PI 650158 genotypes were found to be within acceptable limits in terms of both oil and protein ratios in places with semi-temperate conditions.
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    Exploring differentially expressed genes in Phaseolus vulgaris L. during BCMV infection
    (Academic Press Ltd- Elsevier Science Ltd, 2024) Yeken, Mehmet Zahit; Celik, Ali; Emiralioglu, Orkun; Ciftci, Vahdettin; Baloch, Faheem Shehzad; Ozer, Goksel
    Bean common mosaic virus (BCMV) is a significant pathogen that affects common bean, leading to substantial yield losses and reduced crop quality. To mitigate BCMV attacks, certain genes, including diacylglycerol kinases genes (PvDGKs), genes associated with defense and stress responses (PvGST, PvPAL, PvLOX, and PvPOD), as well as genes related to plant defense (PvPR1, PvPR2, and PvPR3) play an essential functional role in various stress responses in common bean. In this study, the expression levels of PvDGK1, PvDGK2, PvDGK3, PvDGK5a, PvDGK5b, PvDGK6, PvGST, PvPAL, PvLOX, PvPOD, PvPR1, PvPR2, and PvPR3 genes were investigated in the leaves of different common bean genotypes under BCMV infection conditions. Through quantitative real -time PCR analysis, we observed varying expression patterns for all these genes at different time points during viral infection. The tolerant genotype exhibited higher expression levels of all PvDGKs, PvGST, PvPAL, PvPOD, PvPR1, and PvPR2 genes compared to the susceptible genotype, with the PvPR1 gene showing the highest transcript levels. These findings provide the initial evidence of the potential roles of PvDGKs, PvGST, PvPAL, PvLOX, PvPOD, PvPR1, PvPR2, and PvPR3 in responding to the stress induced by BCMV in common bean. The results presented herein will serve as a valuable resource for guiding future breeding studies aimed at addressing BCMV-induced stress in common bean cultivation.