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    Molecular analysis of isoniazid, rifampin and streptomycin resistance in Mycobacterium tuberculosis isolates from patients with tuberculosis in Düzce, Turkey
    (2005) Öztürk, Elif; Sanic, Ahmet; Kaya, Demet; Ceyhan, İsmail
    The aim of this study was to use DNA sequencing analysis to analyze the mutations in the most commonly targeted genes (katG, inhA, rpoB, rpsL) in isoniazid (INH)-, rifampin (RIF)- and streptomycin (SM)-resistant Mycobacterium tuberculosis strains obtained from subjects in Düzce, Turkey. Four isolates were found to be INH-resistant, 3 were RIF- resistant and 5 were SM-resistant, out of a total of 52 M. tuberculosis strains. In 3 of the 4 INH-resistant strains, a mutation in the katG gene in codon 315 appeared as AGC ? ACC (Ser ? Thr), and the other INH-resistant strain showed a mutation in the katG gene in codon 314 as ACC ? CCC (Thr ? Pro). There were no mutations in the inhA gene in INH-resistant isolates. Two of the 3 RIF-resistant strains were found to have mutations in the rpoB gene in codon 516 appearing as GAC ? GTC (Asp ? Val), and the other RIF-resistant strain has a mutation in the rpoB gene in codon 531 as TCG ? TTG (Ser ? Leu). These 3 RIF-resistant strains are also INH-resistant. All 5 SM-resistant strains have mutations in the rpsL gene in codon 43 appearing as AAG ? AGG (Lys ? Arg). Thus, we found common gene mutations that bring about the resistance of M. tuberculosis to antituberculosis drugs in all of our isolates from Düzce. To the best of our knowledge, the ACC ? CCC (Thr ? Pro) mutation in the katG gene in codon 314 has not been previously defined.
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    The resistance to major antituberculous drugs of mycobacterium tuberculosis strains isolated from the respiratory system specimens of tuberculosis patients in Duzce, Turkey
    (Natl Inst Infectious Diseases, 2005) Öztürk, C. Elif; Balbay, Öner A.; Kaya, Demet; Ceyhan, İsmail; Bulut, İsmet; Şahin, İdris
    Though generally curable, tuberculosis (TB) is becoming increasingly resistant to commonly used antibiotics. Drug-resistant and multidrug-resistant (MDR)-TB is a consequence of monotherapy, insufficient drug therapy and national TB control programs. The present study was designed to reveal the resistance to major antimicrobial drugs (isoniazid [INH], streptomycin [SM], ethambutol [EMB], and rifampicin [RIF]) of Mycobacterium tuberculosis isolated from the respiratory specimens of TB patients in Duzce, Turkey. A total of 62 TB patients (46 male, 16 female; age: 17-75 mean: 42 +/- 15.9) were included in the study; 52 (83.8%) were new cases and susceptible to all anti-TB drugs, while 10 (16.2%) were previously treated cases. Antimicrobial susceptibility tests were performed by the proportion method in Lowenstein-Jensen medium. Fifty-two of the 62 (83.8%) isolated M. tuberculosis strains were found to be susceptible to all drugs, and 7 (11.3%), 5 (8%), and 3 (4.8%) were resistant to SM, INH, and RIF, respectively; 3 (4.8%) were MDR. There were no EMB-resistant strains. The results of this study. show the presence of drug-resistant and MDR strains of TB at Duzce in the northwest part of Turkey.