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    Effects of plant growth regulators, different culture media and strength MS on production of volatile fraction composition in shoot cultures of Ocimwn basilicum
    (Elsevier Science Bv, 2018) Monfort, Lucila Elizabeth Fragoso; Bertolucci, Suzan Kelly Vilela; Lima, Andreisa Fabri; de Carvalho, Alexandre Alves; Mohammed, Aliyu
    The influence of different culture media, strength MS media and plant growth regulators (PGR) was assessed through the growth and production of the volatile fraction composition in Ocimwn basilicum (basil). The treatments were: the use of different culture media (MS, B5 and WPM), strength MS media (2MS, MS, 1/2MS and 1/4MS) and different PGR combinations or only IAA, NAA, IBA, BAP and TDZ. The leaf number, nodal segment, shoot number, lenght, shoot dry weight, root number, root size, root dry weight, as well as the volatile fraction analysis through Head-Space - GC/MS were assessed at the 40th culture day. The present results suggest that media type, MS concentration and the growth regulator types have qualitative and quantitatively influenced the growth and volatile composition. A higher amount of methyleugenol in the media 2MS and 1/4MS, and higher content of linalool and 1,8-cineole in MS and WPM were observed. The PGR improved biomass production and induced quantitative modifications in the production of major volatile constituents in Ocimwn basilicum plant lets, although the same major compounds were produced in all treatments. There was a tendency to estragole synthesis in the presence of auxin combined with cytokinin. There was a tendency to linalool synthesis in the presence of auxin isolates.
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    Plantlet regeneration from young leaf segments of Curaua (Ananas Erectifolius), an Amazon species
    (2016) Moreira, Carolina Mariane; Andrade, Helena Botelho De; Bertolucci, Suzan Kelly Vilela; Lameira, Osmar Alves; Mohammed, Aliyu
    The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the highest frequency occurred via culture treatment containing 4-amino-3,5,6-trichloropicolinic acid (picloram; 48.57%), 2,4-D (40%), or TDZ (35.71%). However, only calli generated in treatments containing TDZ or N6-isopentenyladenine (2ip) were able to develop shoots (maximum 35.71% and 14.28%, respectively). A combination of TDZ and 2,4-D improved callus induction (60.0%) but did not increase shooting response. However, both callusing and shooting response increased when 10 days of pulse treatment with TDZ and 2,4-D was applied (66.83% and 48.7%, respectively). Rooted plantlets that exhibited normal growth and development were acclimatized in a greenhouse and had a survival rate of 95%.