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  • Küçük Resim
    Öğe
    Evaluation and comparison of alpha- and beta-amanitin toxicity on MCF-7 cell line
    (Tubitak Scientific & Technical Research Council Turkey, 2014) Kaya, Ertuğrul; Bayram, Recep; Yaykaşlı, Kürşat Oğuz; Yılmaz, İsmail; Bayram, Sait; Yaykaşlı, Emine; Yavuz, Muhsine Zeynep; Gepdiremen, Ali Akçahan
    Background/aim: Alpha- and beta-amanitins are the main toxins of the poisonous Amanita phalloides mushroom. Although there are many studies available concerning alpha-amanitin, there are limited data about beta-amanitin in the literature. Therefore, this study is aimed at comparing the toxic effects of alpha- and beta-amanitin on the MCF-7 cell line. Materials and methods: The alpha- and beta-amanitins used for this research were purified from Amanita phalloides by preparative high-performance liquid chromatography The MCF-7 breast cancer cell line was used, and specific concentrations of the toxins (100, 10, 1, 0.1, and 0.01 mu g/mL) were applied to the cells. The MTT test was performed to determine the level of toxicity, and the quantity of protein in the cell was measured using the biuret test. Results: The alpha-amanitin showed a higher toxicity at 36 h, while the highest inhibition of protein synthesis by the beta-amanitin was observed at 24 h. Conclusion: It was shown that the beta-amanitin may be responsible for toxicity, like alpha-amanitin, in Amanita phalloides mushroom poisoning. The early inhibition of protein synthesis for beta-amanitin might be useful for future experiments and research.
  • Küçük Resim
    Öğe
    The evaluation and comparison of the alpha and beta amanitin toxicity on MCF-7 cell line
    (Current Biology Ltd, 2013) Kaya, Ertuğrul; Bayram, Recep; Yaykaşlı, Kürşat Oğuz; Yılmaz, İsmail; Bayram, Sait; Yavuz, Zeynep; Gepdiremen, Akçahan
  • Küçük Resim
    Öğe
    Purification and in vitro toxicity of gamma amanitin
    (Taylor & Francis Ltd, 2015) Bakırcı, Sinan; Bayram, Recep; Yılmaz, İsmail; Yaykaşlı, Kürşat Oğuz; Bayram, Sait
    We aimed to obtain gamma amanitin with high purity through a purification process and compare toxic effects of alpha, beta, and gamma amanitin. Specific concentrations of the toxins (25, 10, 1, and 0.1 mu g/mL) were applied to the C3A human hepatocytes. A MTT test was performed to determine the level of toxicity. Alpha amanitin showed a higher toxicity in 48 h while the lowest toxicity was observed in beta amanitin. The toxicity level of gamma amanitin was found between the alpha and beta amanitin toxicity. Our method is suitable for obtaining gamma amanitin with high purity (>99%) as well as for obtaining alpha amanitin and beta amanitin. Gamma amanitin has been shown to have equal responsibility for toxicity as alpha amanitin in amanita poisoning.