Yazar "Batool, Neelam" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • Küçük Resim Yok
    Öğe
    In silico designing and characterization of outer membrane protein K (OmpK) from Vibrio anguillarum and its expression in Nicotiana tabacum for the development of a plant-based vaccine against fish vibriosis
    (Elsevier, 2024) Malik, Muhammad Suleman; Elahi, Iqra; Sameeullah, Muhammad; Ijaz, Fatima; Batool, Neelam; Khalid, Fatima; Gurel, Ekrem
    Vibriosis is caused by Vibrio anguillarum in various species of aquaculture. A novel, secure, and stable vaccine is needed to eradicate vibriosis. Here, for reverse vaccinology and plant -based expression, the outer membrane protein K (OmpK) of V. anguillarum was chosen due to its conserved nature in all Vibrio species. OmpK, an ideal vaccine candidate against vibriosis, demonstrated immunogenic, non -allergic, and non-toxic behavior by using various bioinformatics tools. Docking showed the interaction of the OmpK model with TLR-5. In comparison to costly platforms, plants can be used as alternative and economic bio-factories to produce vaccine antigens. We expressed OmpK antigen in Nicotiana tabacum using Agrobacterium-mediated transformation. The expression vector was constructed using Gateway (R) cloning. Transgene integration was verified by polymerase chain reaction (PCR), and the copy number via qRT-PCR, which showed two copies of transgenes. Western blotting detected monomeric form of OmpK protein. The total soluble protein (TSP) fraction of OmpK was equivalent to 0.38% as detected by ELISA. Mice and fish were immunized with plant -derived OmpK antigen, which showed a significantly high level of anti-OmpK antibodies. The present study is the first report of OmpK antigen expression in higher plants for the potential use as vaccine in aquaculture against vibriosis, which could provide protection against multiple Vibrio species due to the conserved nature OmpK antigen.
  • Küçük Resim
    Öğe
    De novo assembly and characterisation of chloroplast genomes of broccoli cvs. Marathon and Green sprout using next generation sequencing
    (Scientific and Technological Research Council Turkey, 2022) Zia, Ayisha; Abdullah, Misbah Zahoor; Nisar, Asma; Batool, Neelam; Bibi, Amana; Gürel, Ekrem
    The genus Brassica (family Brassicaceae) includes nutritionally and economically important species such as Brassica napus, Brassica rapa, and Brassica oleracea. Many varieties of B. oleracea are available in various morphological forms including nutritive vegetables such as cauliflower (var. botrytis), Brussels sprouts (var. gemmifera), kales and collards (var. acephala), kohlrabi (var. gongylodes), cabbage (var. capitata), and broccoli (var. italica). Objective of the present study was to sequence chloroplast genomes of two cultivars of broccoli: Marathon and Green sprout. The sequencing was done by next generation sequencing. The analysis was performed using Velvet, Geneious, GeSeq, tRNAscan-SE, ARAGORN, OrganellarGenomeDRAW, IRscope and REPuter. The genomes of both cultivars showed highly similar quadripartite structure of 153,365 bp. The LSC (83,136 bp) and SSC (17,835 bp) regions were separated by a pair of IR (26,197 bp) region. In total, 114 unique genes were present in both species, including 80 protein-coding, 30 tRNA and 4 rRNA genes, while 18 genes were duplicated in IRs. The highest amino acid encoding frequency was found for Leucine whereas cysteine was the least encoding amino acid. The codon usage analyses confirmed high encoding efficacy of codons that ended at 3'-end with A/T. Repeat analyses of these genomes revealed 415 microsatellites and 36 oligonucleotide repeats. Microsatellites motifs were mostly comprised of A/T instead of C/G. The comparative analyses confirmed the presence of 17 substitutions between both cultivars. Overall, this study will increase knowledge about the chloroplast genomes of broccoli and will provide a resource for chloroplast genetic engineering of this important edible plant.