Yazar "İmren, Mustafa" seçeneğine göre listele
Listeleniyor 1 - 20 / 96
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Adana ilinde önemli kültür bitkilerinde bulunan bitki paraziti nematod türleri1(2014) Kasapoğlu, Ece B.; İmren, Mustafa; Elekçioğlu, İbrahim HalilBitki paraziti nematodlar kültür bitkilerinde önemli ekonomik kayıplara neden olan zararlı organizmalardır. Adana'da tarım yapılan alanlarda bulunan bitki paraziti nematodların saptanması amacıyla 2010 yılında toplam 140 toprak örneği alınmıştır. Bitki paraziti nematodların türleri klasik yöntemlerle (Morfometrik karakterler) teşhis edilmiştir. Tylenchida, Aphelenchida ve Dorylaimida takımlarına ait 8 familyaya bağlı 12 cinse ait 23 bitki parazi nematod türü tespit edilmiştir. Pratylenchus zeae Graham, 1951, P. loosi Loof, 1960, P. delattrei Luc, 1958, Helicotylenchus digonicus Perry, in Perry, Darling and Thorne, 1959, Scutylenchus cylindricaudatus Ivanova, 1968, Türkiye Doğu Akdeniz Bölgesi için yeni kayıttır.Pratylenchus delattrei (Luc), Türkiye nematod faunası için yeni kayıt niteliğindedir. Bu çalışmada bulunan en yaygın türlerin Aphelenchus avenae, Rotylenchulus macrosomus, Pratylenchus thornei, Merlinius brevidens ve Tylenchulus semipenetrans olduğu belirlenmiştir.Öğe Alternative strategies to control root-knot nematodes (Meloidogyne spp.) with different irrigation systems in pepper greenhouses(2015) Toktay, Halil; İmren, Mustafa; Bozbuğa, RefikKök-ur nematodları geniş bir konukçu dizisine sahip olup önemli kültür bitkilerinde ağır verim kayıplarına neden olmaktadır. Türkiye’de nematodlar ile mücadelede nematisit kullanımına önemli kısıtlamalar getirilmekte bunun yanında mücadelede yeni metotlar ortaya konması gerekmektedir. Bu yüzden biber seralarında Kök-ur nematodları ile mücadelede alternatif mücadele programlarının geliştirilmesi büyük önem taşımaktadır. Bu çalışmada toprak solarizasyonu ile birlikte iki farklı kimyasal ve toprak fumigantının iki ve dört hat şeklinde damla sulama sistemi ile kombine edilerek nematodlarla ile mücadele programlarında kullanılabilirliği araştırılmıştır. Çalışmada solarizasyon + metam sodyum + iprodione uygulaması, dört lateral hatlı damla sulama sisteminde verime ve nematodun kökteki urlanması üzerine etkili bulunmuştur. Bitki boyu ve nematodun üreme gücüne solarizasyon + iprodione uygulamasının dört lateral hatlı damla sulama sisteminde en yüksek etkiye sahip olduğu saptanmıştır.Öğe Assessment of biocontrol potential of different Steinernema species and their bacterial symbionts, Xenorhabdus species against larvae of almond moth, Ephestia cautella (Walker)(Pergamon-Elsevier Science Ltd, 2023) Yüksel, Ebubekir; Ormanoğlu, Nesrin; İmren, Mustafa; Canhilal, RamazanThe almond moth, Ephestia cautella is a serious pest of stored food commodities including dried figs, dates, raisins, cocoa beans. The control of E. cautella infestations in storage that mostly relies on toxic insecticides or fumigants has caused emergence of resistant pest populations as well as residues on the commodities. Ento-mopathogenic nematodes (EPNs) and their endosymbionts are one of the emerging approaches in eco-friendly pest management and pose no health risk to humans and the environment. Therefore, here we evaluated the toxicity of different EPN species (Steinernema feltiae, S. carpocapsae, and S. bicornotum) and their endosymbiotic bacteria (Xenorhabdus nematophila, X. bovienii, and X. budapestensis) on the 3rd and 4th instars larvae of E. cautella under controlled conditions. The IJs of EPNs were applied to concrete arenas in Petri dishes at three concen-trations (50, 100, and 200 IJs/cm2). Oral and contact toxicity of the cell-free supernatants and cell suspension of symbiotic bacteria (4x107 cells/mL) were tested against both larval instars of E. cautella. Steinernema carpocapsae and S. feltiae induced mortalities over 80% at their lowest concentrations against both larval instars after 24 h of application. Oral and contact treatments of cell-free supernatants and cell suspensions were lethal for both larval instars; however, higher mortalities were generally obtained from direct exposure of larvae to cell-free super-natants and cell suspensions. The contact treatment of X. nematophila exhibited the highest virulence, causing 70 and 82.5% mortalities in 3rd and 4th instar larvae after 72 h of application, respectively. The anti-feedant ac-tivity of cell-free supernatants and cell suspensions was also observed in both instars in oral treatments. The findings of this study suggest that EPNs and their symbiotic bacteria have enormous potential for the eco-friendly control of E. cautella.Öğe Assessment of the natural presence of entomopathogenic nematodes in Kayseri soils, Turkey(Springer, 2017) Canhilal, Ramazan; Waeyenberge, Lieven; Yüksel, Ebubekir; Koca, Abdurrahman Sami; Deniz, Yunus; İmren, MustafaEntomopathogenic nematodes (EPNs) have lots of ideal attributes as biological control agents, such as a wide host range, rapid host mortality, ease of commercial production in vivo or in vitro, active host-seeking ability, long-term efficacy, easy application, compatible with many chemical pesticides and environmental safety. In the present study, 61 EPNs isolates from 174 soil samples were collected for the districts of Kayseri Province and from 5 different habitats (Forest, pasture, field crops, vegetable, and fruit orchards). The positive soil samples ratio was (35.1%). Forty-seven of isolates were Steinernema with the ratio of (27%). Prevalence of Heterorhabditis was (8%) (14 isolates). Nematodes were identified by morphometric and molecular methods. Forty-one of these isolates, were identified as Steinernema feltiae. Two isolates were S. carpocapsae, one was S. bicornutum, 6 were unknown Steinernema spp., and 14 were Heterorhabditis bacteriophora. Of these nematodes, S. bicornutum was a first record in Turkey. The soil textures of the EPN positive soils were loam, sandy loam, loamy sand and sandy clay loam with weakly basic (pH 7.57-7.91) and 1.15-3.06% organic content. The electrical conductivity of the nematode positive soils varied from 0.19 to 0.79 (none saline) dS/m.Öğe Association analysis of resistance to cereal cyst nematodes (Heterodera avenae) and root lesion nematodes (Pratylenchus neglectus and P-thornei) in CIMMYT advanced spring wheat lines for semi-arid conditions(Japanese Soc Breeding, 2016) Dababat, Abdelfattah A.; Ferney, Gomez-Becerra Hugo; Erginbaş-Orakcı, Gül; Dreisigacker, Susanne; İmren, MustafaTo identify loci linked to nematode resistance genes, a total of 126 of CIMMYT advanced spring wheat lines adapted to semi-arid conditions were screened for resistance to Heterodera avenae, Pratylenchus neglectus, and P. thornei, of which 107 lines were genotyped with 1,310 DArT. Association of DArT markers with nematode response was analyzed using the general linear model. Results showed that 11 markers were associated with resistance to H. avenae (pathotype Ha21), 25 markers with resistance to P. neglectus, and 9 significant markers were identified to be linked with resistance to P thornei. In this work we confirmed that chromosome 4A (similar to 90-105 cM) can be a source of resistance to P. thornei as has been recently reported. Other significant markers were also identified on chromosomal regions where no resistant genes have been reported for both nematodes species. These novel QTL were mapped to chromosomes 5A, 6A, and 7A for H. avenae; on chromosomes 1A, 1B, 3A, 3B, 6B, 7AS, and 7D for P. neglectus; and on chromosomes 1D, 2A, and 5B for P. thornei and represent potentially new loci linked to resistance that may be useful for selecting parents and deploying resistance into elite germplasm adapted to regions where nematodes are causing problem.Öğe Bazı ekmeklik buğday çeşitlerinin kök yara nematodları Pratylenchus thornei ve Pratylenchus neglectus'a (Tylenchida: Pratylenchidae) karşı dayanıklılıklarının belirlenmesi(2015) İmren, Mustafa; Elekcioğlu, İbrahim Halil; Özkan, HakanKök yara nematodları, Pratylenchus spp. bitkilerin kökünde beslenir ve toprak kökenli patojenlerin bitkiye girişini sağlayan yaralanmalara sebep olur. Buğday yetiştiriciliğinde nematodlara karşı en etkin mücadele yöntemi dayanıklı/ tolerant çeşitlerin kullanımı olarak bilinmektedir. Bu çalışmada, Türkiye’de yetiştiriciliği yapılan 82 adet ekmeklik buğday çeşidinin kök yara nematodları, Pratylenchus thornei ve P. neglectus’a karşı in vitro koşullarda dayanıklılıkları araştırılmıştır. Çalışma sonucunda denemeye alınan buğday genotiplerinden P. thornei ve P. neglectus’a karşı dayanıklı çeşide rastlanılmamıştır. Bununla birlikte, P. thornei’ye karşı 29 çeşit orta dayanıklı, 44 çeşit orta hassas ve 9 çeşidin hassas olduğu ve P. neglectus’a karşı ise 37 çeşit orta dayanıklı, 38 çeşit orta hassas ve 7 çeşidin hassas olduğu saptanmıştır. Ayrıca, 14 adet buğday çeşidi hem P. thornei’ ye hem de P. neglectus’a karşı orta derecede dayanıklı bulunmuştur. Denemeye alınan çeşitler içerisinde Uzunyayla, Atlı, Yayla 305 ve Harmankaya buğday çeşitleri CROC_1/AE. SQUARROSA(224)//OPATA) buğday çeşidine göre daha yüksek performans gösterdiği için ulusal ıslah programlarına dahil edilmesi önerilmektedir.Öğe Characterization of potato golden cyst nematode populations (globodera rostochiensis) in Turkey(Friends Science Publ, 2020) Toktay, Halil; Evlice, Emre; İmren, Mustafa; Özer, Göksel; Ali, Muhammad Amjad; Dababat, AbdelfattahGolden potato cyst nematode, Globodera rostochiensis (Wollenweber) Behrens, is one of the most important soilborne pathogens causing economic losses in potato. The nematode is known to occurin several countries including Turkey and has a worldwide regulatory concern. In this study, identification and genetic diversity of G. rostochiensis specimens obtained from the main potato producing areas of Turkey were determined. Twenty -five of 35 soil samples collected from the provinces of Izmir, Nevsehir and Nigde were found to contain G. rostochiensis. The variation between G. rostochiensis populations was determined when examined according to ecological and pathogenic characteristics of nematode in Turkey. The cysts of G. rostochiensis were identified by measuring the morphological characters using perennial patterns, vulval cone, vulval basin and juveniles. Phylogenetic analysis of the Large Sub Unit (LSU) region of rDNA sequences was used to assess the inter or intra phylogenetic relationships between the nematode populations. The phylogenetic analyses demonstrated that the nematode specimens from Turkey cluster with Globodera spp. and signified the presence of single species of G. rostochiensis. As a result, morphological, morphometric and molecular methods were successfully combined for identification and characterization of G. rostochiensis. The frequency of G. rostochiensis in regulatory samples frompotato -producing areas is becoming increasingly important. The morphological characterization has several complications in the detection of this quarantine nematode, using of this combination is beneficial for a reliable and quick diagnostic for these nematodes which is crucial for regulatory services and growers. The results might help to investigate different ecotypes of G. rostochiensis for comprehensive understanding about physiology, ecology , and biology of the genus Globodera for its effective management in Turkey. (C) 2020 Friends Science PublishersÖğe The complex cell wall composition of syncytia induced by plant parasitic cyst nematodes reflects both function and host plant(Frontiers Media Sa, 2017) Zhang, Li; Lilley, Catherine J.; İmren, Mustafa; Knox, John Paul; Urwin, Peter E.Plant-parasitic cyst nematodes induce the formation of specialized feeding structures, syncytia, within their host roots. These unique plant organs serve as the sole nutrient resource for development and reproduction throughout the biotrophic interaction. The multinucleate syncytium, which arises through local dissolution of cell walls and protoplast fusion of multiple adjacent cells, has dense cytoplasm containing numerous organelles, surrounded by thickened outer cell walls that must withstand high turgor pressure. However, little is known about how the constituents of the syncytial cell wall and their conformation support its role during nematode parasitism. We used a set of monoclonal antibodies, targeted to a range of plant cell wall components, to reveal the microstructures of syncytial cell walls induced by four of the most economically important cyst nematode species, Globodera pallida, Heterodera glycines, Heterodera avenae and Heterodera filipjevi, in their respective potato, soybean, and spring wheat host roots. In situ fluorescence analysis revealed highly similar cell wall composition of syncytia induced by G. pallida and H. glycines. Both consisted of abundant xyloglucan, methyl-esterified homogalacturonan and pectic arabinan. In contrast, the walls of syncytia induced in wheat roots by H. avenae and H. filipjevi contain little xyloglucan but are rich in feruloylated xylan and arabinan residues, with variable levels of mixed-linkage glucan. The overall chemical composition of syncytial cell walls reflected the general features of root cell walls of the different host plants. We relate specific components of syncytial cell walls, such as abundant arabinan, methyl-esterification status of pectic homogalacturonan and feruloylation of xylan, to their potential roles in forming a network to support both the strength and flexibility required for syncytium function.Öğe Correction to: Molecular characterization of Heterodera cruciferae Franklin, 1945 from cabbage felds in Nigde province, Turkey (Sep, 10.1007/s11033-022-07860-w, 2022)(Springer, 2023) Toktay, Halil; Akyol, Badel G.; Evlice, Emre; İmren, MustafaIn the original publication of the article, the affiliation of the third author was incorrectly published.Öğe Corrigendum to “Potential of four Turkish isolates of entomopathogenic nematodes against three major stored products insect pests” [J. Stored Prod. Res. 83 (September 2019) 317–321] (Journal of Stored Products Research (2019) 83 (317–321), (S0022474X19302826), (10.1016/j.jspr.2019.08.003))(Elsevier, 2021) Yüksel, Ebubekir; Canhilal, Ramazan; İmren, MustafaThe authors regret that the following statement was not added inadvertently to the footnote of the original manuscript when published online. < This article was produced from a master thesis carried out in 2017>. The authors would like to apologise for any inconvenience caused.Öğe The cyst nematode Heterodera latipons Franklin, 1969 (Nemata: Heteroderidae): hatching behavior and development on wheat in arid and semiarid regions in Turkey(2020) İmren, MustafaThe cyst nematode Heterodera latipons Franklin, 1969 (Nemata: Heteroderidae) is an economically important sedentary plant-parasitic nematode which limits the production of cereal crops around the world. It is an important pest on wheat and can be found globally in different growing areas, especially in the Mediterranean region. Developing an understanding of its physiological behavior (senescence, quiescence, and cryptobiosis), development [second-stage juveniles (J2) and immature and mature females], and population dynamics under field conditions is needed to create an appropriate control strategy. The experiments were conducted at Karaisalı, a semiarid region in Adana Province, and at Derik, an arid region in Mardin Province, during the 2015–2016 and 2016–2017 growing seasons. The results indicated that the emergence of J2 of H. latipons in the soil rhizosphere occurred from late December to mid-January in the semiarid region, while they were mostly observed through March in both regions when the average temperature was around 10 °C. The white (immature) females were seen between mid-February and mid-March in both experimental seasons in the arid and semiarid regions, and they were mainly observed in April when the temperature was above 15 °C during both seasons in the arid region. The results showed that H. latipons produces only one generation in each wheat growing season, and the developmental stages of this nematode species are closely related to the ecological and edaphic factors of host plant development. Moreover, the development of J2 and immature females of H. latipons took longer in the semiarid region than in the arid region owing to plant and root growth and edaphic factors, especially soil temperature and moisture. Understanding the physiological behavior and biological stages of this nematode will be useful for developing nematode control strategies to keep H. latipons populations below economic damage levels.Öğe Dayanıklılık geni Cre1’in Akdeniz tahıl kist nematodu, Heterodera latipons Franklin (Tylenchida: Heteroderidae)’e karşı etkinliğinin araştırılması(2014) İmren, Mustafa; Uludamar, Ece Börteçine Kasapoğlu; Dababat, Abdelfattah; Toktay, Halil; Elekcioğlu, İbrahim HalilTahıl kist nematodları (Heterodera avenae group) buğdayın önemli zararlılarından biri olup, Türkiye’de Heterodera avenae Wollenweber, H. latipons Franklin ve H. filipjevi (Madzhidov) Stone olmak üzere üç önemli türü farklı buğday üretim alanlarında saptanmıştır. Ülkemizin makarnalık buğdayın merkezi olan Güneydoğu Anadolu Bölgesi’nde Akdeniz tahıl kist nematodu, Heterodera latipons’un yaygın olarak bulunduğu, bölge genelinde birçok lokasyonda ekonomik zarar eşiğinin üstünde popülasyona sahip olduğu rapor edilmiştir. Buğday yetiştiriciliğinde kist nematodları ile en etkin mücadele yöntemi dayanıklı/tolerans çeşit kullanımı olarak bilinmektedir. Günümüzde buğdayın yabani türlerinden kültür formlarına aktarılmış dokuz farklı dayanıklılık geni (Cre genleri) mevcuttur. Bu çalışmada dayanıklılık geni Cre1’in H. latipons’un Gaziantep (Karkamış) popülasyonuna karşı etkinliği araştırılmıştır. Çalışmada genomunda dayanıklılık geni Cre1 bulunan SILVERSTAR buğday çeşidinin yanı sıra SOKOLL, CALINGIRI, GOLDMARKER, FRAME, STYLET, PASTOR ve CROC_1/AE.SQUARROSA(224)//OPATA çeşitlerine ait ebeveynlerin melezlenmesi ile geliştirilen 38 adeti dayanıklılık geni taşıyan, 26 adeti dayanıklılık geni taşımayan toplam 64 adet buğday genotipi H. latipons’a karşı denemeye alınmıştır. Çalışma sonucunda 64 adet genotipten 20’si nematoda dayanıklı, 32’si orta dayanıklı ve 12’sinin ise orta hassas olduğu tespit edilmiştir. Genomunda Cre1 geni taşıyan 38 melezden ise 10’nun H. latipons’a karşı dayanıklı, 20’sinin orta dayanıklı ve 8’nin ise orta hassas olduğu saptanmıştır. Bu çalışma ile dayanıklılık geni Cre1’in H. latipons’un Gaziantep (Karkamış) popülasyonuna karşı mutlak bir dayanıklılığa sahip olmamakla birlikte %78 etkinlik oranı ile başarılı olduğu ve ulusal buğday ıslah programlarında H. latipons’a karşı kullanılabileceği sonucuna varılmıştır. Ayrıca, PASTOR, SOKOLL ve CROC_1/AE.SQUARROSA(224)//OPATA buğday genotiplerinin H. latipons’a karşı iyi bir dayanıklılık kaynağına sahip olduğu saptanmıştır.Öğe Detection of Bursaphelenchus mucronatus associated with acanthocinus griseus on pinus nigra in the Northwestern Turkey(Organization of Nematologists of Tropical America, 2019) Tülek, Adnan; Kepenekçi, İlker; İmren, Mustafa; Akbulut, Süleyman; Tülek, Betül; Koca, Abdurrahman Sami; Özdikmen, HüseyinA survey was conducted in Edirne Province, Turkey for the pinewood nematode, Bursaphelenchus xylophilus. Wood samples were collected from declining black pine trees. Nematodes were extracted from wood samples and insect specimens were collected. Nematodes were observed and identified microscopically and molecularly with the internal transcribed spacer (ITS) of ribosomal DNA. Bursaphelenchus xylophilus was not detected, but B. mucronatus was found in very high numbers with an average up to 300 nematodes/g of wood shaving. Acanthocinus griseus (Coleoptera: Cerambycidae) was detected to be associated with B. mucronatus as a potential vector. This is the first report of B. mucronatus association with A. griseus in Turkey. © 2019 Organization of Nematologists of Tropical America. All rights reserved.Öğe Determination of plant parasitic nematodes in potato growing areas in Bolu province(2018) İmren, MustafaPotato (Solanum tuberosum L.) is one of the most important crops grown for food in the world, but many potato-yield limiting biotic factors are present such as plant parasitic nematodes (PPN). This study was conducted to determine the nematodes associated with potato growing areas during 2014-2016 growing seasons in Bolu province. Overall, Root lesion nematode, Pratylenchus spp. were the most common nematode genus occurring in potato production fields with an incidence of over 52.5%. The Cyst nematode, Globodera spp. was found in 35% and Root knot nematode, Meloidogyne spp. was in 20% of the samples whereas potato cropping very frequently with low rotation, incidences of plant-parasitic nematodes were 62.5% of Ditylenchus spp., 50% Geocenamus spp., 40% for Helicotylenchus spp. or Rotylenchus spp., 30% for Paratylenchus and Amplimerlinus spp., 9% for Pratylenchoides spp. and Tylenchorhynchus spp. The survey results suggested that the presence and the abundance of plant-parasitic nematodes were found relatively high in potato growing areas in Bolu and may have a potential to cause dramatic yield losses. The comprehensive surveys are indispensable to define the distribution, frequency as well as more accurate identification of plant parasitic nematodes species, particularly Globodera spp., Meloidogyne spp. and Pratylenchus spp. species in potato growing areas of Bolu province.Öğe Determination of the genetic variation within the egg parasitoid, trichogramma brassicae, bezdenko (Hymenoptera: Trichogrammatidae) populations in Düzce province, Turkey(2018) Koca, Abdurrahman Sami; İmren, Mustafa; Kütük, HalilMembers of Trichogramma genus are the most widely used as natural enemies in biological control programs. Choice of natural enemies is known to have a critical important for the succession of biological control. Their morphologic identification has some bottlenecks as laborious, time-consuming and mostly based on very small structures of the bodies. Molecular techniques have been widely used for detection, discrimination, phylogenetic analysis, identification and characterization in biological control agents belong to Trichogramma spp. In this study, the efficacy of the direct sequencing analysis of internal transcribed spacer locus of rDNA was investigated for analyzing the genetic variation within nine Trichogramma brassicaepopulation from corn growing areas in Düzce province. The comparison of the sequences was performed with other ITS2 locus of Trichogramma species available in GenBank database. The result of analyses indicated that no genetic variation was determined in the Düzce population.Öğe Determining the optimal Meloidogyne Incognita inoculum level, inoculation time, pathogencity and gall development on tomato roots for resistance experiments in breeding programs(Society for Plant Research, 2015) Bozbuğa, Refik; İmren, Mustafa; Kasapoglu, Ece Bortecinec; Toktay, Halil; Elekcioglu, İbrahim HalilRoot knot nematodes (RKN) cause a significant damage to many cultivated crops and present study was conducted to determine the most appropriate inoculum levels of a ubiquitous RKN nematode in warm climate, Meloidogyne incognita, inoculation time and plant age for the screening of nematode resistance studies on plant breeding programs under controlled conditions. The inoculum level experiment was conducted to investigate the effect of four M. incognita inoculum levels (1000, 1500, 2000, 2500 juveniles/ 1000 gram soil mix) on three different age of susceptible tomato plants. Gall development was observed at 3 different ages of susceptible tomato plants with 7 different time points. The highest gall numbers were observed on nematode infected 3 day old plant roots and the least number of galls were detected on the roots of 5 day old plants. First galls were observed at 1 dpi and the highest gall number was observed on the 21st gall counting day on three day old tomato roots. Thus the inoculation of 2000- 2500 second stage juveniles /1000 gram soil mix to 3 days old tomato plants was the best to reach high gall numbers which are suitable for screening of nematode resistance studies on plant breeding programs. © 2015 Society for Plant Research. All rights reserved.Öğe Determining the resistance of some bread wheat varieties against root lesion nematodes: Pratylenchus thornei and Pratylenchus neglectus (Tylenchida: Pratylenchidae)(Ankara Univ, Fac Agr, 2015) İmren, Mustafa; Elekcioğlu, İbrahim Halil; Özkan, HakanRoot lesion nematodes, Pratylenchus spp. feeds on plants root and causes wounds provide a means of entry for soilborne pathogen. It is known that the using of resistant/tolerant wheat varieties is the most effective method in order to control nematodes in wheat cultivation. In this study, the resistance of 82 bread wheat varieties grown in Turkey were investigated against root lesion nematodes, Pratylenchus thornei and P. neglectus, in-vitro conditions. At the end of this study, no resistant wheat genotype against Pratylenchus thornei and P. neglectus was found. However, it was found that 29 varieties moderately resistant, 44 varieties moderately susceptible, 9 varieties susceptible against P. thornei and 37 varieties moderately resistant, 38 varieties moderately susceptible, 7 varieties susceptible against P. neglectus. Additionally, 14 wheat varieties were moderately resistant against both P. thornei and P. neglectus. Among the tested wheat varieties, Uzunyayla, Atli, Yayla 305 and Harmankaya showed higher performance than CROC_1/AE. SQUARROSA(224)//OPATA) variety, therefore it is suggested that these varieties should be incorporated into national wheat breeding programs.Öğe Distribution and occurrence of plant-parasitic nematodes associated with raspberry (Rubus idaeus) in Souss-Massa region of Morocco: Relationship with soil physico-chemical factors(Russian Acad Sci, Inst Parasitology, 2019) Mokrini, Fouad; Laasli, Salah-Eddine; Iraqui, Driss; Wifaya, Ahmed; Abdelaziz, Mimouni; Orakçı, Gül Erginbaş; İmren, MustafaRaspberry (Rubus idaeus) polytunnels in Morocco's Souss-Massa region were surveyed between February and April 2018. The study was aimed to investigate the diversity and incidence of plant-parasitic nematodes affecting raspberry crop and to assess the effects of soil physico-chemical properties on the nematodes. Twelve nematode genera were identified form the soil and root samples collected from 41 raspberry polytunnels across the three provinces (Belfaa, Biougra and Khmis Ait Aimra). The most common plant-parasitic nematodes (PPN) were Pratylenchus spp., Meloidogyne spp. and Helicotylenchus spp. In terms of their abundance and frequency, four PPN (Pratylenchus spp., Meloidogyne spp., Helicotylenchus spp. and Tylenchus spp.) were abundant and frequent throughout the region. Several genera of nematodes were significantly associated with soil texture, organic matter and pH, which indicate that soil properties play an important role in PPN communities. This description of PPN assemblages associated with red raspberry polytunnels in Souss-Massa region provides a starting point from which further studies will be implemented for the other regions of Morocco cultivating raspberries, and to develop efficient management strategies.Öğe Distribution of Steinernematids and Heterorhabditids (Rhabditida: Steinernematidae and Heterorhabditidae) in the Southern Anatolia Region of Turkey(Springer, 2016) Canhilal, Ramazan; Waeyenberge, Lieven; Toktay, Halil; Bozbuğa, Refik; Çetintaş, Ramazan; İmren, MustafaThis study was conducted to isolate and identify entomopathogenic nematode (EPN) species in different agricultural areas in the Southern Anatolia region of Turkey. Out of 193 soil samples, 20 (10.33%) turned out to be positive for the presence of EPNs, belonging to the genera Steinernema and Heterorhabditis. Morphological and molecular (ITS-RDNA) characterizations were performed for the 20 EPN isolates. Six isolates were identified as H. bacteriophora, 2 as H indica and 6 as S. feltiae. Two isolates were undescribed Steinernemas pecies. H. indica was recorded for the first time in the southern Anatolia region of Turkey. Molecular analysis confirmed the morphological identification of Heterorhabditidae and Steinernematidae species. Comparative analysis of the ITS sequences revealed interspecific polymorphism among the tested isolates. Additionally, intraspecific polymorphism was observed within S. feltiaeand H bacteriophorapopulations, but no intraspecific polymorphism was detected in the H indicapopulation.Öğe Diversity and identification of plant-parasitic nematodes in wheat-growing ecosystems(MDPI, 2022) Keçici, Ayça İrem; Bozbuğa, Refik; Öcal, Atilla; Yüksel, Ebubekir; Özer, Göksel; Yıldız, Şenol; İmren, MustafaSeveral nematode species can be found in different densities in almost any soil ecosystem, and their diversity in those ecosystems depends on numerous reasons, such as climatic conditions and host presence. Cereals are one of the main hosts of plant-parasitic nematodes (PPN), chiefly root-lesion nematodes (RLN, Pratylenchus spp.) and cereal cyst nematodes (CCN, Heterodera spp.). These nematodes are known as major parasites of the cereal crops; however, agricultural areas accommodate various nematodes showing biological variation. The diversity of parasitic nematodes on cereals in the Sakarya provinces of Turkiye, where cereals are intensively grown and located in the middle of two climatic zones, has not been well studied. Therefore, in this study, we aimed to determine the diversity, identification, and molecular phylogeny of PPNs in wheat-growing ecosystems in the Hendek, Pamukova, Geyve, Akyazi, and Central districts of Sakarya. The diversity of PPNs was calculated using the Shannon diversity index. Thirteen PPN genera were detected in 92% of soil samples. Heterodera filipjevi was identified in 24% of the soil samples using morphological, morphometrical, and molecular tools. In the morphological and molecular analyses, intraspecific polymorphism was observed in H. filipjevi populations. The result indicated that the high infestation rate of H. filipjevi was recorded from Geyve and Pamukova, followed by Hendek and Akyazi; however, a low infestation rate was detected in the Central district. The moderate value of the Shannon index of migratory nematode species was obtained in wheat fields as 2.31, whereas the value of evenness was 0.93, implying moderate diversity and high evenness of nematodes. This study is the first comprehensive report on H. filipjevi from wheat cropping areas in the Sakarya province. Intensified cereal cropping systems with/without non-cereal rotations increased the risk of plant-parasitic nematodes, especially RLNs and H. filipjevi infection of wheat production areas in the province.
