Yazar "Ülgen, Canan" seçeneğine göre listele
Listeleniyor 1 - 6 / 6
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Certain chemical, physical and microbiological properties of the freeze-dried (lyophilized) apple of cain/cane apple(Bolu Abant İzzet Baysal Üniversitesi, 2013) Ülgen, Canan; Yurdugül, SeyhunKocayemiş (Arbutus unedo), Türkiye'nin Karadeniz bölgesinde ve dünyada daha çok ılıman iklimlerde yetişen yenilebilir bir meyvedir. Bu meyvenin bazı tıbbi özellikleri vardır. Bunlar antidiyabetik, idrar söktürücü, tansiyon düşürücü, idrar yolu temizleyici ve iltihap sökücü gibi özelliklerdir. Bu çalışmada taze ve dondurulup kurutulmuş Arbutus unedo meyvesinin belirli fiziksel, kimyasal ve antimikrobiyal özellikleri araştırılmıştır. Arbutus unedo'nun dondurup kurutulmuş biçiminin E.coli, S.aureus, S.epidermidis ve Salmonella spp. bakterileri üzerinde duyarlı olduğu bulunmuştur. Ek olarak kurutulmuş ve taze meyve arasında pH, renk, toplam çözünmüş katı madde miktarı, vitamin C içeriği, ağırlığı, kül miktarı, tat testi ve SEM analizleri gibi bazı deneylerde çok önemli farklar görülmemiştir.Öğe Do magnetic field applications affect in vitro regeneration, growth, phenolic profiles, antioxidant potential and defense enzyme activities (SOD, CAT and PAL) in lemon balm (Melissa officinalis L.)?(Elseiver, 2021) Ülgen, Canan; Yıldırım, Arzu BirinciMelissa officinalis L. (lemon balm) is a valuable medicinal plant used in the treatment of nervousness, cardiac failure, hypersensitivities, depression, insomnia, indigestion and colic. Objective of the first part of the study was to determine the effect of 3 different magnetic fields (MF-50 mT, 100 mT and 150 mT) with 5 different exposure times (5 min, 15 min, 30 min, 1 h and 3 h) on in vitro regeneration of explants containing preexisting meristem (axillary, shoot tip and hypocotyl buds). Regenerated shoots were also compared in terms of shoot length, root length, fresh-dry weight and % water content. In the second stage, regenerated shoots obtained from 3 different MF treatments for 1 h and no MF-treated shoots (control) were compared according to their phenolic profile, total phenol-flavonoid content, free radical scavenging activity, superoxide dismutase (SOD), catalase (CAT) and phenylalanine ammonium lyase (PAL) activities. The best regeneration was obtained with axillary buds exposed to 100 mT for 1 h (15.8 shoots at 100 % shoot frequency). Furthermore, 50 mT and 100 mT MF treatments improved the growth parameter and water contents of regenerated shoots. Comparison of 4 different sources (3 MF exposure and no exposure-control) found out that 50 mT MF treatment was the most effective for the enhancement of rosmarinic acid quantity, total phenol-flavonoid content, non-enzymatic antioxidant activity (IC50), stress related enzymatic antioxidant activity (SOD and CAT) and phenol production related PAL enzyme activity. Although 100 mT MF for 1 h treatment was found to be the most potent in regard to number of shoot formation, the most productive shoot length and fresh-dry weight was obtained with 50 mT MF exposure for 1 h. Considering the all results about growth parameters, medicinal quality (rosmarinic acid amount, total phenol-flavonoid content and antioxidant activity) and endurance capacity against stress (SOD, CAT and PAL activities), it can be concluded that exposure to 50 mT MF was the most effective treatment for in vitro culture of M. officinalis.Öğe Effect of magnetic field treatments on seed germination of melissa officinalis L.(2017) Ülgen, Canan; Yıldırım, Arzu Birinci; Türker, Arzu UçarMelissa officinalis L., commonly known as lemon balm, is a perennial herb belonging to Lamiaceae family. It has therapeutic properties, such as sedative, carminative, antispasmodic, anti-viral, wound healing, digestive, diuretic, diaphoretic, anti-septic and anti-thyroid. Lemon balm has been used for the treatment of headache, indigestion, colic, nervousness, cardiac problems, depression, rheumatism, indigestion, hypersensitivities, anxiety and depression traditionally. Beneficial effects of lemon balm are ascribed to the phenolic compounds such as rosmarinic acid, tannins and flavonoids. Studies showed that magnetic field applications in agriculture can be used to improve the quality and quantity of the product. Positive effects of the stationary magnetic field on the plant seed germination have been recorded with some plant species. In this study, effects of magnetic field on M. officinalis seeds were investigated. Seeds were sterilized in 0.1 % HgCl2 for 10 min and 70 % Ethanol for 1-2 min. After surface sterilization of the seeds, they were placed in petri dishes containing Murashige and Skoog's medium with sucrose and agar. Neodymium block magnets (100 X 50 X 5 mm) were used to create magnetic fields (50 mT and 100 mT). Ten seeds were placed in each petri plates and 10 petri plates were used for each treatment. Petri dishes containing surface sterilized seeds were placed in 3 different conditions [without magnetic field application (control) and magnetic field applications (low-50 mT and high-100 mT)] and the germination (radicle protrusion) was assessed. Seeds were exposed to magnetic fields for 1, 3, 6, 12, 24, 48, 72, 144 and 240 hours. The number of germinated seeds was recorded for 20 days. Best seed germination was obtained with 100 mT magnetic field application for 1 hour (52 %). Seed germination rate was rather low (28 %) without magnetic field application (control). In consistent with 100 mT magnetic field application, 1 hour exposure to 50 mT magnetic field gave better germination rate (36 %) than control. The lowest seed germination was observed with 240 hours exposure to both magnetic fields (27 % for 100 mT and 16 % for 50 mT). Magnetic field applications also decreased the seed germination time. Although seed germination was observed in 11. day with control, it was obtained in 7. day with both magnetic field applications. Magnetic field application enhanced the percentage of germinated seed and shortened the period of seed germination in M. officinalis.Öğe Effects of magnetic field on tissue culture, biological activity, phenolic content and defense enzyme activities (sod, cat and pal) of Melissa officinalis L. (Lemon balm), a medicinal plant(Bolu Abant İzzet Baysal Üniversitesi, 2022) Ülgen, Canan; Türker, ArzuMelissa officinalis L. (limon balsamı), geleneksel tıpta depresyon, sinirlilik, kaygı, aşırı duyarlılık, baş ağrısı, kolik, hazımsızlık, kalp sorunları, romatizma ve uykusuzluk tedavisinde kullanılan değerli bir şifalı bitkidir. Çalışmanın amacı, değişen maruz kalma sürelerine sahip üç farklı manyetik alanın (MA-50 mT, 100 mT ve 150 mT) tohum çimlenmesini ve in vitro (sürgün ucu, aksiller ve hipokotil tomurcukları) eksplant büyümesini nasıl etkilediğini değerlendirmektir. MA uygulaması yapılmış in vitro çoğaltılan sürgünler ve fideler de sürgün ve kök uzunluğu, yaş ve kuru ağırlık ve su içeriği yüzdesi dikkate alınarak değerlendirilmiştir. Ayrıca, 3 farklı MA uygulamasına 1 saat maruz bırakılarak ve MA maruziyeti olmadan (kontrol) elde edilen in vitro çoğaltılmış sürgünler, bireysel fenolik içerik, toplam fenol ve flavonoid içeriği, biyolojik etkiler (antibakteriyel ve antioksidan) ve enzim aktiviteleri [süperoksit dismutaz (SOD), katalaz (CAT) ve fenilalanin amonyum liyaz (PAL)] bakımından da değerlendirilmiştir. Değerlendirilen tüm MA uygulamaları (50 mT, 100 mT ve 150 mT), tohum çimlenme yüzdesini iyileştirdi ve tohum çimlenme uzunluğunu kısalttı. Ayrıca, MA uygulanmış tohumlardan yetiştirilen fidelerin kontrol fidelerinden (MA maruziyeti yok) daha uzun kök ve sürgün uzunluklarına ve daha fazla su içeriğine sahip olduğu bulunmuştur. En iyi rejenerasyon, aksiller tomurcuklar 1 saat boyunca 100 mT'ye maruz bırakıldığında meydana gelmiştir (15.8 sürgün, %100 sıklıkta). Ek olarak, düşük ve orta MA uygulamaları (50 mT ve 100 mT), in vitro çoğaltılan sürgünlerin su içeriğinin yanı sıra büyüme parametrelerini de desteklemiştir. Buna karşılık, rosmarinik asit miktarı, toplam fenol ve flavonoid içeriği, antioksidan aktiviteler [enzimatik (SOD ve CAT) ve enzimatik olmayan (IC50)] ve fenol sentezi ile ilgili PAL enzim aktivitesi 50 mT MA ile en yüksek olandır. Antibakteriyel kapasite, yalnızca MA maruziyeti olmayan sürgünlerde gözlemlenmiştir. En etkili sürgün uzunluğu ve taze-kuru ağırlık, 1 saat süreyle 50 mT MA uygulaması ile elde edilmiştir, ancak 1 saat süreyle 100 mT MA'ın in vitro sürgün yayılımı açısından en etkili olduğu belirtilmiştir. Büyüme parametreleri, tıbbi öz (rosmarinik asit miktarı, toplam fenol-flavonoid içeriği ve antioksidan potansiyel) ve PAL, SOD ve CAT seviyelerine ilişkin stresle başa çıkma kapasitesileri ile ilgili tüm bulgular dikkate alındığında, 50 mT MA uygulamasının in vitro olarak M. officinalis yayılımı için en uygun yaklaşım olduğu sonucuna varılmıştır.Öğe Enhancement of plant regeneration in lemon balm (Melissa officinalis L.) with different magnetic field paplications(2020) Ülgen, Canan; Yıldırım, Birinci Arzu; Türker, Arzu UçarMelissa officinalis L. (lemon balm) is a valuable medicinal and aromatic plant in the Lamiaceae family. Two independent experiments were performed to improve the productivity of the plant regeneration. Firstly, the most efficient in vitro culture system of M. officinalis was determined using 7 different explant types (leaf, petiole, stem, root, axillary buds, shoot buds and cotyledon buds) on medium containing Murashige and Skoog minimal organics (MSMO) medium with different concentrations and combinations of plant growth regulators (PGRs). Micropropagation was obtained only with explants containing meristematic cells (axillary buds, shoot tip buds and cotyledon buds). Lemon balm had a very low regeneration capacity and in the second part of the experiment, enhancement of regeneration was aimed with the applications of different magnetic fields (MFs). Two different MFs (50 and 100 mT) were generated using neodymium block magnets. There was no MF exposure with control treatment. Three different explants (axillary, shoot tip and cotyledon buds) were cultured on media including BA in combination with indole-3-acetic acid (IAA) or naphthalene acetic acid (NAA) with the application of two different MFs at 1 hour duration. As a result, it was determined that MF applications enhanced the regeneration capacity of M. officinalis and the best shoot formation was observed with axillary bud explant cultured in 1.5 mg/L BA at 100 mT MF application for 1 hour duration.Öğe Impacts of three different magnetic field applications on seed germination and seedling development of Melissa officinalis L.(2024) Ülgen, Canan; Türker, Arzu UçarThe herb Melissa officinalis L., most commonly recognized as lemon balm, has been used as a treatment for problems with the brain and central nervous system, headaches, nervousness, digestive disorders, respiratory and circulatory conditions, various types of cancer and rheumatism. This investigation was designated to compare the impacts of 3 discrete magnetic field strengths (50 mT, 100 mT and 150 mT) with various time periods of exposure (5 min, 15 min, 30 min, 1 hr and 3 hr) on M. officinalis seed germination (rate and onset) and seedling growth (length of shoot-root, weight of fresh-dry and water content). The seeds, which were surface sterilized in petri plates, were subjected to different applications, including exposure to various magnetic fields and control (without exposure). Germination was assessed by observing the radicular protrusion. The number of germinated seeds was recorded over 20 days. In all 3 magnetic field applications, exposure to magnetic field for 1 hr caused the highest number of seed germination (36%, 52% and 50% for 50 mT, 100 mT and 150 mT, respectively) comparing to control (28%). The onset of seed germination was earlier with magnetic field application (day 7) compared to control (day 11). Growth elements and water contents of the seedling were assessed in order to understand the impact of different magnetic field applications. Implementation of 100 mT and 150 mT magnetic field for 1 hr increased shoot and root length in addition to fresh weight of the seedlings. The maximum dry weight increase was provided by 150 mT-5 min, 15 min and 30 min. The highest water content (%) was observed with exposure to 150 mT (68%) and 100 mT (66%) for 1 hr compared to control (44%). Magnetic field intensity of 100 mT and 150 mT for 1 hr was the most efficient in the seed germination and seedling growth.
