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    Identification and genetic diversity of the Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) in cereal production areas of Northern Cyprus
    (Entomological Soc Turkey, Ege Univ, 2020) Özarslandan, Adem; İmren, Mustafa; Özer, Göksel; Karaca, Cem; Dababat, Abdelfattah
    The Mediterranean cereal cyst nematode, Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae) is a destructive plant-parasitic nematode on cereal crops in particularly wheat and barley. It has a global distribution with a severe negative impact on yield quantity. In this study, a survey was conducted to identify plant-parasitic nematodes in cereal-growing areas in Cyprus. Forty-five samples including roots and soil from the root zone of plants were collected from cereal fields located in Gazimagusa, Gime, Guzelyurt and Lefkosa Provinces before crop harvesting from late-May and early-June in 2017. Cyst-forming nematodes were determined by Fenwick's flotation and decanting techniques from 37 soil samples (82%). The internal transcribed spacer (ITS) regions of the ribosomal DNA of isolates were amplified and sequenced and subjected to a BLASTn search of the NCBI database for species identification, and the analyses showed that all samples were identified as H. latipons. Phylogenetic analyses based on ITS sequences revealed that H. latipons isolates from Northern Cyprus were closely related to isolates obtained from Morocco, Russia, Syria and Turkey. Data of this study demonstrated for the first time the presence of H. latipons in the cereal fields of Gazimagusa, Gime, Guzelyurt and Lefkosa Provinces, where the nematode most likely causes serious economic problems in the cereal production. These results were the most up-to-dated analyses on the occurrence of H. latipons in cereal fields of Northern Cyprus and provided basic data for breeding programs to improve the resistant levels in the local cultivars.
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    A study on the presence of potato cyst nematodes: globodera rostochiensis (Wollenweber, 1923) Skarbilovich, 1959 and Globodera Pallida Stone 1973 (Tylenchida: Heteroderidae) in Nevşehir province, Turkey
    (2019) Özarslandan, Adem; Dinçer, Dilek; Bozbuğa, Refik; İmren, Mustafa; Elekçioğlu, İbrahim Halil
    Potato (Solanum tuberosum L.) cyst nematodes (Globodera rostochiensis (Wollenweber) and Globodera pallida (Stone) cause significant yield losses and have been listed as quarantine pests for many countries in the world including Turkey, and the European and Mediterranean Plant Protection Organization (EPPO, A2). In Turkey, the current potato production area is decreasing due to contamination by quarantine agents, and new fields are under threat of invasion by new quarantine organisms. In this study, samples were collected from Nevşehir province, Turkey, in potato growing fields during the spring growing season in 2014-2015. Soil samples were washed using Fenwick’s can and cysts were extracted and identified using molecular markers. The species-specific molecular markers generated a 435 bp using PITSr3, PITSp4 and ITS5 primers. The potato golden cyst nematode, G. rostochiensis was identified in Nevşehir potato production areas and this is the first report for the Central Anatolia Region. In addition, G. rostochiensis was found in two samples of the same field in the surveys. The average cyst nematode density was determined as 60 cysts in 250 g soil.
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    Türkiye buğday faunası için yeni bir tür, Meloidogyne artiellia (Franklin)’nın morfolojik ve moleküler yöntemlerle tanımlanması
    (2014) İmren, Mustafa; Özarslandan, Adem; Kasapoğlu, Ece B.; Toktay, Halil; Elekçioğlu, İbrahim Halil
    Kök-ur nematodları (Meloidogyne spp.) dünyada ekonomik önem sahip bitki paraziti nematodları arasındadır. Meloidogyne cinsine ait nematod türleri sebzelerde ve tahıllarda önemli oranda ürün kayıplarına neden oldukları bilinmektedir. Kök ur nematodu, M. artiellia özellikle tahıllar, baklagiller ve cruciferae familyasına ait ürünlerde zarara neden olabilmektedir. Bu çalışmadaki M. artiellia izolatları Türkiye’de ilk defa Hatay ili buğday ekim alanlarından 2012 yılı üretim sezonunda toplanan toprak ve kök örneklerinden elde edilmiş olup morfolojik ve moleküler olarak tanımlamaları yapılmıştır. M. artiellia, ikinci dönem larva ve dişi bireylere ait morfolojik özellikler ve allometrik kriterler esas alınarak tanımlanmıştır. Ayrıca, mitokondriyal DNA’nın sitokrom C oksidaz alt ünitesine (COI) ait nükleotid dizilimleri kullanılarak moleküler olarak da tanımlanmış ve diğer kök ur nematodu türlerinden bariz olarak ayrıldığı saptanmıştır.